. The actinomycetes. Actinomycetales. 170 THE ACTINOMYCETES, Vol. I 90. o S. fradia* o S. gritaut N. polychromog«n«* N. a«(«roid«> 30 40 50 TIME (MINUTES) 60 Figure 73. Lysis of cell walls of Streptomyces vs. Nocardia by lysozynie (Reproduced from: Romano A. H. and Sohler, A. J. Bacteriol. 72: 866, 1956). at 30 or 37°C, lysis takes place in 4 to 6 hours. Not all the mycelium is lysed uni- formly, some of the hyphae producing chlamydospores, spherical bodies, or other fragmentary material. Under favorable con- ditions, these bodies are able to grow and develop into fresh colonies. Ka
. The actinomycetes. Actinomycetales. 170 THE ACTINOMYCETES, Vol. I 90. o S. fradia* o S. gritaut N. polychromog«n«* N. a«(«roid«> 30 40 50 TIME (MINUTES) 60 Figure 73. Lysis of cell walls of Streptomyces vs. Nocardia by lysozynie (Reproduced from: Romano A. H. and Sohler, A. J. Bacteriol. 72: 866, 1956). at 30 or 37°C, lysis takes place in 4 to 6 hours. Not all the mycelium is lysed uni- formly, some of the hyphae producing chlamydospores, spherical bodies, or other fragmentary material. Under favorable con- ditions, these bodies are able to grow and develop into fresh colonies. Katznelson isolated from manure com- posts a culture of a thermophilic actinomy- cete, whi(!h grew well on organic media. When transferred to a synthetic medium con- taining ammonium sulfate and starch, it un- derwent lysis after 24 to 48 hours of incuba- tion at 50°C. During growth, the culture became acid. Addition of CaCOs to the cul- ture prev(!ntod lysis. Stanier reported that an agar-decomposing strain of S. coelicolor un- derwent rapid autolysis and soon died out. Lysis took place when the organism entered the sporulating stage. The process of autolysis in cultures of S. griseus, the streptomycin-producing organ- ism, has been studied extensively. When the growth of this organism in submerged culture reaches a maximum, lysis sets in and frag- mentation of the mycelium occurs. The peak of streptomycin production lags somewhat behind the growth peak. The changes in re- action of medium take place in two phases. One occui;s during growth, when the medium becomes acid, and one is associated with ly- sis of the culture, when the medium becomes alkaline and may reach a pH of Dulaney et al. (1947) differentiated two phases of metabolic activity of S. griseus, grown inider shaken conditions:. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may n
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