. Carnegie Institution of Washington publication. BLACK ROT OK CRUCIFEROUS PLANTS. 327 the end of 1, 2, 5, io, and 15 minutes. Lysol in per cent solution killed in 1 minute, but in per cent solution it failed to kill in 15 minutes. Carbolic acid in per cent solu- tion killed in 5 minutes, but not in 2 minutes. The organism is quite sensitive to the presence of sodium chloride, but not as much so as Bacterium phaseoli. It grew very feebly in salted gelatin (o gelatin converted into + 26 by adding HC1). Further comparisons should be made. The organism is able to live in mixed
. Carnegie Institution of Washington publication. BLACK ROT OK CRUCIFEROUS PLANTS. 327 the end of 1, 2, 5, io, and 15 minutes. Lysol in per cent solution killed in 1 minute, but in per cent solution it failed to kill in 15 minutes. Carbolic acid in per cent solu- tion killed in 5 minutes, but not in 2 minutes. The organism is quite sensitive to the presence of sodium chloride, but not as much so as Bacterium phaseoli. It grew very feebly in salted gelatin (o gelatin converted into + 26 by adding HC1). Further comparisons should be made. The organism is able to live in mixed cultures for a considerable time (Russell, Smith). In agar cultures at room-temperatures Harding states that the organism remained alive from 4 to 6 months. In the cool-box on potato the writer has kept it alive for a year (average temperature about 120 C). Harding injected fresh bouillon cultures ( cc. subcutaneously, 2 cc. intravenously, and 4 cc. subperitoneally) into rabbits with no ill effect other than a temporary loss in weight. RESUME OF SALIENT CHARACTERS. Positive. Pathogenic to Cruciferae, dissolves middle lamella, plugs vessels, forms numerous closed cavities in the host-plant; in cabbage causes conspicuous black stain in veins of leaf. vShort rods with rounded ends, single or in pairs, and occasionally in short chains of 4 or more; sometimes much resembling a coccus (Coccobacillus) when crowded in the plant or in old cultures; sometimes slightly curved or irregular in shape. Long chains or non- septate filaments frequently occur in sugar-rich media. Pseudozoogloeae; yellow on all media, changing to dirty yellow-brown in the plant and on cruciferous substrata (culture media); motile in newly diseased tissues and in young cultures, 1-flagellate; very resistant to drying under certain conditions. Surface colonies on agar or gelatin rather slow- growing, circular, pale yellow at first, deepening with age, smooth, wet-shining, flat, with distinct margin; buried colo
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