Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . ourth neutral in re-action. Partly fill a beaker of medium size with water andplace it within a second larger beaker which also containswater, the two vessels being separated by pieces of cork. Fasten the four test-tubes com-pactly together by means of arubber band, lower them intothe water of the inner beakerand suspend them, by means ofa clamp attached to one of thetubes, in such a manner thatthe albumin solutions shall bemidway between the upper
Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . ourth neutral in re-action. Partly fill a beaker of medium size with water andplace it within a second larger beaker which also containswater, the two vessels being separated by pieces of cork. Fasten the four test-tubes com-pactly together by means of arubber band, lower them intothe water of the inner beakerand suspend them, by means ofa clamp attached to one of thetubes, in such a manner thatthe albumin solutions shall bemidway between the upper andlower surfaces of the water. Inone of the tubes place a ther-mometer with its bulb entirelybeneath the surface of the al-bumin solution (Pig. 19). Gentlyheat the water in the beakers,noting carefully any changeswhich may occur in the albuminsolutions and record the exacttemperature at which thesechanges occur. The first appear-ance of an opacity in an albuminsolution indicates the commence-ment of coagulation and the tem-perature at which this occursshould be recorded as the coagu-lation temperature for that par-ticular albumin Coagulation TemperatureApparatus. What is the order in which the four solutions coagulate? 1ROTEIDS. 5 I Repeat the experiment adding to the first tube I drop ofacetic acid, to the second i drop of concentrated KOH solu-tion, to the third 2 drops of a 10 per cent NaCl solution andleave the fourth neutral as before. What is the order of coagulation here? Why? 14. Precipitation by Alcohol.— Prepart 3 test tubes eachcontaining about of 95 per cent alcohol. To the first addone drop of per cent HC1, to the second one drop of KOHsolution and leave the third neutral in reaction. Add to eachtube a few drops of egg albumin solution and note the do you conclude from this experiment? Alcohol pre-cipitates proteids unaltered but if allowed to remain underalcohol the proteid is coagulated. The fixing of tissuesfor histological
Size: 1019px × 2454px
Photo credit: © The Reading Room / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1900, bookpublisherphiladelphiablakis
