. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS 343 channel for 16 channels, with true 1 MS switching rate between channels). High resolution images of the myocyte cultures were recorded with a computer-based image acquisition system (Macintosh, equipped with a Data Translation framegrabber) connected to the video camera. Cell cultures. Cultures were established according to conven- tional techniques ( reference 4 for details). The cultures were kept in an incubator at 35°C in a water-saturated atmosphere of ambient air
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS 343 channel for 16 channels, with true 1 MS switching rate between channels). High resolution images of the myocyte cultures were recorded with a computer-based image acquisition system (Macintosh, equipped with a Data Translation framegrabber) connected to the video camera. Cell cultures. Cultures were established according to conven- tional techniques ( reference 4 for details). The cultures were kept in an incubator at 35°C in a water-saturated atmosphere of ambient air and CO2. Patterned growth was obtained by plating the cells onto coverslips precoated with patterns of a light-sensitive resin (photoresist) that rejected adhesion of cells ( reference 4 for details). During the experiments, the cultures were mounted in a temperature-controlled experimental cham- ber and superfused at 5 to 10 ml/min with Hank's balanced salt solution (HBSS) at 35°C, pH After the extracellular stim- ulating electrodes had been placed, the cells were stained with 60 to 80 Mg/ml of the voltage sensitive dye DI-8-ANEPPS (5) for 5 min. A typical experiment is illustrated in Figure 1. The recording was obtained from a 2-day-old culture. The cell strand was stim- ulated at Hz with an extracellular electrode delivering ms wide square pulses at twice threshold intensity. The electrode was placed at a sufficient distance from the recording site that only propagated action potentials could invade the region of interest. Recordings were made with a 100X, objective providing a spatial resolution in the object plane of 15 X 15 nm for each detector element. Panel A shows the positions of the photodiode array pixels with respect to the outlines of the in- DKECTION OF PROPAGATION. 1 0 TIME [msl DETECTOR ELEMENT « Figure I. Discontinuous action potential propagation along a chain ol single ventricular myocytes in culture. (A) Outlines, ba
Size: 2367px × 1056px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
