. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Plate Cultures 207 ever, that it would be a great omission not to describe the original method in detail. Apparatus.—Half a dozen glass plates, measuring about 6 by 4 inches, free from bubbles and scratches and ground at the edges, are carefully cleaned, placed in a sheet-iron box made to receive them, and sterilized in the hot-air closet. The box is kept tightly closed, and in it the sterilized plates can be kept indefinitely before use. A moist chamber
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Plate Cultures 207 ever, that it would be a great omission not to describe the original method in detail. Apparatus.—Half a dozen glass plates, measuring about 6 by 4 inches, free from bubbles and scratches and ground at the edges, are carefully cleaned, placed in a sheet-iron box made to receive them, and sterilized in the hot-air closet. The box is kept tightly closed, and in it the sterilized plates can be kept indefinitely before use. A moist chamber, or double dish, about 10 inches in diameter and 3 inches deep, the upper half being just enough larger than the lower to allow it to close over it, is carefully washed. A sheet of bibulous paper is placed in the bottom, so that some moisture can be retained, and a i : 1000 bichlorid of mercury solu- tion poured in and brought in contact with the sides, top, and bottom by turning the dish in all directions. The solution is emptied out, and the dish, which is kept closed, is ready for use. A leveling apparatus is required. It consists of a wooden tripod with ad- justable screws, and a glass dish covered by a flat plate of glass upon which a low bell-jar stands. The glass dish is filled with broken ice and water, covered with the glass plate, and then exactly leveled by adjusting the screws under the legs of the tripod. When level, the cover is placed upon it, and it is ready for use. Method.—A sterile platinum loop is dipped into the material to be examined, a small quantity secured, and stirred about so as to distribute it evenly throughout the contents of a tube of melted gelatin. If the material under examination be very rich in bacteria, one loopful may contain a million individuals, which, if spread out in a thin layer, would develop so many colonies that it would be impossible to see any one clearly; hence further dilation becomes nec- essary. From the first tube, the
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1919
