. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 556 KENXEY, BELAMARICH AND SHEPRO of 14 ± 5 HIM ) are often as effective as 17 ± 6 inn EDTA in inhibiting amebocyte aggregation for 5 minutes but EDTA at final concentrations of 17 ± 6 IHM is em- ployed routinely. The transistory nature of EDTA inhibition eliminates the possibility of employ- ing standardized cell pools of known amebocyte number in aggregation experi- ments, for even if EDTA preparations are kept on ice with very gentle agitation to prevent sedimentation, most of the amebocytes are in small to medium sized a
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 556 KENXEY, BELAMARICH AND SHEPRO of 14 ± 5 HIM ) are often as effective as 17 ± 6 inn EDTA in inhibiting amebocyte aggregation for 5 minutes but EDTA at final concentrations of 17 ± 6 IHM is em- ployed routinely. The transistory nature of EDTA inhibition eliminates the possibility of employ- ing standardized cell pools of known amebocyte number in aggregation experi- ments, for even if EDTA preparations are kept on ice with very gentle agitation to prevent sedimentation, most of the amebocytes are in small to medium sized aggregates within 15-20 minutes. Effect of calcium and I/UKJUCSIUIII of amebocytes in EDTA Calcium and magnesium produce aggregation of amebocytes in EDTA (Fig. 4). Minimum final concentrations of 8 ± 1 imr calcium or magnesium cause only slight a o 1001 90- 80- 70- 60- 50-. 40- 30- 20-^ 10- 60 75 90 105 120 TIME (sec) FIGURE 5. Effect of serum, AHS and cell free plasma on amebocytes in EDTA. Data from experiments on 3 different animals. Control aggregation in saline (•). Amebocytes in 17 ± 6 HIM EDTA (O) ; amebocytes in EDTA with 1:20 diluted AHS (A); amebocytes in EDTA wtih 1:20 diluted serum ('A); amebocytes in EDTA with 1:20 diluted cell free plasma ( • ).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
