. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CALCIFICATION IN SEA URCHIN SPINES 153 care being taken not to touch the regenerated skeleton. The disc, of the same diameter as the planchet, was cut from the cuff of a rubber autopsy glove and pierced with a hot pin. Spines of different diameter were shielded by discs with holes of appropriate size. Each spine was counted for three minutes and the activity expressed as counts per minute per spine. In some of the experiments, 7 10 s 'o x I 4 O •^ 3 (_) O o 2 in 0 A B C. 0 Time After Fracture (days) FIGURE 1. Kinetics of 45C
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CALCIFICATION IN SEA URCHIN SPINES 153 care being taken not to touch the regenerated skeleton. The disc, of the same diameter as the planchet, was cut from the cuff of a rubber autopsy glove and pierced with a hot pin. Spines of different diameter were shielded by discs with holes of appropriate size. Each spine was counted for three minutes and the activity expressed as counts per minute per spine. In some of the experiments, 7 10 s 'o x I 4 O •^ 3 (_) O o 2 in 0 A B C. 0 Time After Fracture (days) FIGURE 1. Kinetics of 45Ca incorporation by regenerating spines of five specimens of S. pitrpnratits (A-E) incubated in vivo for five days following experimental fracture. Each point represents the mean value of 3 or 5 spines, with vertical bars indicating the range of all values. Control values are means of 3 bleached spines. activity of 45Ca in the incubation medium and coelomic fluid was determined in triplicate, 50 microliter aliquots which were dried on planchets and counted for three minutes. This activity was expressed as counts per minute per milliliter. All counts were corrected for background. In experiments on the effects of temperature and Diamox on calcification rates, activity was determined independently by liquid scintillation techniques using the sample preparation method of Carr and Parsons (1962), adapted here to the assay. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
