. The principles of bacteriology: a practical manual for students and physicians. wenty-four hours. 164 BACTERIOLOGY. If by then all the staining-material has been dissolved,more should be added, the bottle being again shakenand allowed to stand for another twenty-four hours;this must be repeated until a permanent sediment ofundissolved coloring-matter is seen upon the bottomof the bottle. The bottles are then to be labelled saturated alcoholic or watery solution of fuch-sin, gentian-violet, or methylene-blue, as the case maybe. The alcoholic solutions are not diredly employed forstaining-purp
. The principles of bacteriology: a practical manual for students and physicians. wenty-four hours. 164 BACTERIOLOGY. If by then all the staining-material has been dissolved,more should be added, the bottle being again shakenand allowed to stand for another twenty-four hours;this must be repeated until a permanent sediment ofundissolved coloring-matter is seen upon the bottomof the bottle. The bottles are then to be labelled saturated alcoholic or watery solution of fuch-sin, gentian-violet, or methylene-blue, as the case maybe. The alcoholic solutions are not diredly employed forstaining-purposes. The solutions with which staining is accomplishedare made from the stock solutions in the followingway: An ordinary test-tube of about 13 mm. diameter isthree-fourths filled with distilled water and the con-centrated alcoholic or watery solution of the dyeadded, little by little, until one can just see throughthe solution. It is then ready for use. Care must betaken that the color does not become too dense. Thebest results are obtained when this dilution is just Fig. Back of bottles for staining-solutious. transparent as viewed through a layer about 12 to14 mm. thick. A solution consisting of 5 parts of the ORDINARY STAINING-SOLUTIONS. 165 saturated alcoholic solution and 95 parts of distilledwater proves, as a general thing, to be satisfactory. The above represent the staining-solutions in every-day use. They may be kept in bottles supplied withstoppers and pipettes (Fig. 35), and when used aredropped upon the preparation to be stained. For certain bacteria which stain only imperfectlywith these simple solutions it is necessary to employsome agent that will increase the penetrating action ofthe dyes. Experience has taught us that this can beaccomplished by the addition to the solutions of smallquantities of alkaline substances, or by dissolving thestaining-materials in strong watery solutions of eitheraniline oil or carbolic acid, instead of water—in otherwords, b
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