. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 2. Aggregation assays using Micrvcitma cells prepared from suspensions rotated in CMFSW or CMF - SO4. Assays were earned out in calcified dilutions of MAF prepared as described! 12. 16). Photograph depicts results at 1 h. Wells from left to right in rows 1 and 2 depict serial dilutions of MAF in CMFSW with CaCK. Wells at bottom contain samples of each cell preparation in calcified CMFSW. Reading from the top from left to right: first row, assay contains cells pre-treated in CMFSW; second row. cells pre-treated in CMF
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 2. Aggregation assays using Micrvcitma cells prepared from suspensions rotated in CMFSW or CMF - SO4. Assays were earned out in calcified dilutions of MAF prepared as described! 12. 16). Photograph depicts results at 1 h. Wells from left to right in rows 1 and 2 depict serial dilutions of MAF in CMFSW with CaCK. Wells at bottom contain samples of each cell preparation in calcified CMFSW. Reading from the top from left to right: first row, assay contains cells pre-treated in CMFSW; second row. cells pre-treated in CMF - SO.,. Aggregation is impaired in cells which had been pre-treated in CMF - SO4. quots of cells were then placed in even suspension in the following media: CMFSW, CMF - SO,, MBLSW, MBL - SO4. Each suspension was gently pipetted with a Pasteur pipet and counted; the count in each suspension was ad- justed to 107/rnl. Thirty ml of suspension were then poured into large glass petri dishes and gently pipetted to assure an even distribution of cells. The dishes were then covered and were permitted to remain undisturbed for 24 h at 22°C. Inspection at the end of this period revealed aggregates of varying sizes in samples that had been sus- pended in MBLSW and CMFSW. Aggregation was not observed in cell suspensions lacking sulfate (Fig. 3). ing in single cells and aggregates when sulfate was absent, whether Ca:+ and Mg2" were present (Fig. 4E-F). Our general impression was that processes were most often seen in relatively agranular cells of intermediate to large size. Incorpt >ral it >n of ifSO4 The results of 35SO4 uptake into Microciona cells are shown in Figure 5. Each sampling time point depicts the average of duplicate tests, and the values are expressed as Microscopic studies The most striking observation was a relative lack of cellular blebbing or of nlopodia or pseudopodia in cell preparations suspended in sulfate-free seawater. In con- trast, we commonly observed
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
