. The Canadian field-naturalist. Natural history. 1995 Ramamoorthy and Chinnappa: Sand Dune Long-Stalked Chickweed 217. Figure 1. Capsules of Stellaria longipes. A. Straw-colored, B. Black-colored. system), followed by acetone:HCI (5:1), and 1% HCl in water (Arditti and Dunn 1969). Isolated spots were eluted from the cellulose with 1% HCl in methanol and analyzed using the BAW system described previously, or n-butanol:2N HCl (1:1). Other solvents described by Harbome (1967) were employed but did not produce satisfactory separation of the pigments. These solvents included t- Butanohacetic acid:
. The Canadian field-naturalist. Natural history. 1995 Ramamoorthy and Chinnappa: Sand Dune Long-Stalked Chickweed 217. Figure 1. Capsules of Stellaria longipes. A. Straw-colored, B. Black-colored. system), followed by acetone:HCI (5:1), and 1% HCl in water (Arditti and Dunn 1969). Isolated spots were eluted from the cellulose with 1% HCl in methanol and analyzed using the BAW system described previously, or n-butanol:2N HCl (1:1). Other solvents described by Harbome (1967) were employed but did not produce satisfactory separation of the pigments. These solvents included t- Butanohacetic acid:water (3:1:1), 15% acetic acid, formic acid:water:HCl (5:3:2), and 1% HCl in water. Anthocyanidins were characterized by the Rf values of both the isolated spots and the spots from the ini- tial separation using three systems, spectral analysis of the isolated compounds, and the color of the spots in UV light (Arditti and Dunn 1969). Results and Discussion Pigment contents of the fourteen populations are summarized in Table 2. Cyanidin, malvidin and lute- olinidin were the only anthocyanidins evident in capsules of Stellaria longipes. Black capsules con- tained all three anthocyanidins with cyanidin in the largest proportion. Brown capsules contained more malvidin than cyanidin yet had the same proportion Table 1. List of populations used in the present study, collection sites and their ploidy. Accession Number Collection Site Ploidy 4716 223 569 530 4766 817 133 113 214 3867 798 7332 7294 879 Waterton National Park, Alberta Nose Hill, Alberta Longview, Alberta Exshaw, Alberta Yellowstone National Park, Wyoming Hawk Hills, Northwest Territories Lake Athabasca Sand Dunes, Saskatchewan Cypress Hills, Alberta Little Buffalo Falls, Northwest Territories Rocky Mountain National Park, Colorado Beartooth Pass, Wyoming Quirpon, Newfoundland In = 52 2n = 78 2n=104. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectnaturalhistory, booky
