. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. ASIATIC CHOLERA AND THE CHOLERA ORGANISM 583 periods without passage through the animal body have a tendency to lose the curve, assuming a more bacillus-Hke appearance. The spirilla are stained with all the usual aqueous anilin dyes. They are decolor- ized by Gram's method. In histological section they are less easily stained, but may be demonstrated by staining with alkaline methylene blue. Cultivation.—^The cholera spfrillum grows easily upon all the usual culture media, thriving upo
. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. ASIATIC CHOLERA AND THE CHOLERA ORGANISM 583 periods without passage through the animal body have a tendency to lose the curve, assuming a more bacillus-Hke appearance. The spirilla are stained with all the usual aqueous anilin dyes. They are decolor- ized by Gram's method. In histological section they are less easily stained, but may be demonstrated by staining with alkaline methylene blue. Cultivation.—^The cholera spfrillum grows easily upon all the usual culture media, thriving upon meat-extract as well as upon meat-infusion. •«. ^J'l^^ Fig. 126.—Choiera Spirillum. (After Frankel and Pfeiffeii) media. Moderate alkalinity of the media is preferable, though slight acidity does not prevent growth. In gelatin plates growth appears at room temperature within twenty- four hours as small, strongly refracting yellowish-gray, pin-head colonies. As growth increases the gelatin is fluidified. Under magnification these colonies appear coarsely granular with margins irregular because of the liquefaction. Liquefaction, too, causes a rapid development in such colonies of separate concentric zones of varying refractive powei:. Old strains, artificially cultivated for long periods, lose much of their liquefying power. In gelatin stab cultures fluidification begins at the surface, rapidly giving rise to the famUiar funnel-shaped excavation. Upon agar plates, within eighteen to twenty-four hours, grayish, opalescent colonies appear, which are easily differentiated by their. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Hiss, Philip Hanson, 1868-1913; Zinsser, Hans, 1878-1940. joint author. New York and London, D. Appleton and Company
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