. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 404 .1. <i. MORIN AND G. T. REYNOLDS in distal parts of the upright. Also a short side branch is usually marked by a concentration of photocytes, while long side branches show a distribution similar to the primary upright. The number of photocytes per "segment" (one "segment" as defined here includes a pedicel, node and proximal internode ) in uprights from three different colonies is plotted in Figure 4. The variation is considerable, but the generaliza- tion that photocytes are more concentrated dist
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 404 .1. <i. MORIN AND G. T. REYNOLDS in distal parts of the upright. Also a short side branch is usually marked by a concentration of photocytes, while long side branches show a distribution similar to the primary upright. The number of photocytes per "segment" (one "segment" as defined here includes a pedicel, node and proximal internode ) in uprights from three different colonies is plotted in Figure 4. The variation is considerable, but the generaliza- tion that photocytes are more concentrated distally is FIGURE 5. Fluorescence microscope photograph of a freeze-dried cross-section of a por- tion of an upright containing a photocyte (arrow) in the endoderm. Note the long projec- tion of the photocyte toward the coelenteron (central hollow). The endoderm, ectoderm and perisarc are easily distinguished. Scale bar indicates 20 n. FIGURE 6. Fluorescence microscope photograph of a living Ohclia bicuspidata showing the concentration of photocytes at the tip of the pedicel. Bar indicates 100 ,u. Intermittent examination of an upright over a period of several hours showed that the photocytes did not move a visible distance in that time. Location of pliotocytcs within the endoderm. The observations of living whole mounts indicated that the cells do not lie in the relatively transparent ectoderm (Fig. 3A, B). Fluorescence microscopic examination of freeze-dried, serial sec- tioned material showed that the photocytes are located in the endoderm (Fig. 5). A reconstruction from serial sections showed a correspondence of the green fluores- cent cells seen histologically to the photocyte positions shown in a fluorescence. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods
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