A treatise on the principles and practice of medicine . ught in contact with a typhoidserum. The agglutinins are produced by the tissues reacting to thebacilli. Gruber pointed out the value of the test in proving the existenceof a previous typhoid, while Widal applied the laboratory facts at thebedside. Technique.—A bouillon culture, not more than eighteen hours old,is used, taking forty parts of the bouillon and one of the blood (dilutionof 1 to 40), since the test is quantitative rather than qualitative. Thetest may be made macroscopically in the test-tube, or on the microscopicslide, where
A treatise on the principles and practice of medicine . ught in contact with a typhoidserum. The agglutinins are produced by the tissues reacting to thebacilli. Gruber pointed out the value of the test in proving the existenceof a previous typhoid, while Widal applied the laboratory facts at thebedside. Technique.—A bouillon culture, not more than eighteen hours old,is used, taking forty parts of the bouillon and one of the blood (dilutionof 1 to 40), since the test is quantitative rather than qualitative. Thetest may be made macroscopically in the test-tube, or on the microscopicslide, where immobilization, clumping and agglutination of the bacillimay be observed, sometimes in a very short time—an hour is the usualtime-limit for the test. Ficker employs dead cultures with equallyaccurate results, and this method is more convenient and safe than theuse of living bacilli (Plate,I). Significance.—The reaction may be found thirty years after a typhoidattack. It is sometimes absent in typhoid, particularly in severe cases, PLATE I FIG. 1. Bouillon Culture of Typhoid Bacilli before the Addition of DilutedTyphoid Serum. X 500. (After Cabot.) Serum diagnosis. FIG. 2
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Keywords: ., bookcentury1900, bookdecade1910, bookpublisherphiladelphialeafeb
