. Pathological technique; a practical manual for workers in pathological histology and bacteriology. Fig. 41.—Diplococcus pneumoniae ; cover-glass preparation from the heartsblood of a rabbit; x iooo (Frankel and Pfeiffer). Blood-serum.—Minute colorless, transparent colonies, re-sembling very small drops of dew (Fig. 42). Glycerin Agar-agar.—Feeble growth of very minute gray-ish colonies. Bouillon.—Clouded faintly. Litmus Milk.—Sometimes turned pink and on other culture-media is very feeble. The organ-ism dies out rapidly in cultures. To keep it viable it shouldbe transplante


. Pathological technique; a practical manual for workers in pathological histology and bacteriology. Fig. 41.—Diplococcus pneumoniae ; cover-glass preparation from the heartsblood of a rabbit; x iooo (Frankel and Pfeiffer). Blood-serum.—Minute colorless, transparent colonies, re-sembling very small drops of dew (Fig. 42). Glycerin Agar-agar.—Feeble growth of very minute gray-ish colonies. Bouillon.—Clouded faintly. Litmus Milk.—Sometimes turned pink and on other culture-media is very feeble. The organ-ism dies out rapidly in cultures. To keep it viable it shouldbe transplanted every forty-eight hours. 266 PATHOLOGICAL TECHNIQUE. Pathogenesis.—The pneumococcus is very pathogenic formice and rabbits, less so for guinea-pigs. Subcutaneous inoculation with virulent cultures causes thedeath of mice in from twenty-four to thirty-six hours, andof rabbits in from thirty-six to forty-eight hours, with septi-cemia. This infection is the sputum-septicemia of the autopsy there will be found in the blood everywherethe characteristic encapsulated lancet-s


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