. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EXCYSTMENT OF C. LINGUA 373 induce excystment within a physiologically significant period, but at 37° C. the usual course of excystment was observed (Fig. 2). Pretreatment with gastric conditions followed by those of the small intestine proved most effective in stimulating excystment. Again, this effectiveness was not related directly to temperature or pH. Pretreatment at pH 2 (Ringer-HCl) for 30 minutes failed to stimulate excystment in 1% trypsin, and excystment did not occur in Ringer at pH 7 after 30 minutes pretreatment
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EXCYSTMENT OF C. LINGUA 373 induce excystment within a physiologically significant period, but at 37° C. the usual course of excystment was observed (Fig. 2). Pretreatment with gastric conditions followed by those of the small intestine proved most effective in stimulating excystment. Again, this effectiveness was not related directly to temperature or pH. Pretreatment at pH 2 (Ringer-HCl) for 30 minutes failed to stimulate excystment in 1% trypsin, and excystment did not occur in Ringer at pH 7 after 30 minutes pretreatment in 1% pepsin. Pretreat- 100 CO >t CJ X 60 20. */0 Bile salt 15 45 Minutes 75 FIGURE 4. Effect of bile salt on excystment. Conditions: 30 minutes in 1% pepsin (pH 2) at 37° C., removed to 1% trypsin plus the concentration of sodium taurocholate shown (pH 7). ment with pepsin initiated excystment in trypsin (Table II), and enhanced the rate of excystment in trypsin with other substances and under different physical conditions (Fig. 3). Maximum excystment () was attained within 60 minutes in trypsin-bile salt with cysteine as reducing agent. A carbon dioxide atmosphere was not required for maximum excystment. Sodium taurocholate was not essential for excystment, but it enhanced the effect obtained with trypsin. Its effect was related to its concentration. Initially, the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
