. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. 366 INFECTIVE DISEASES. In sections of the intestine their presence may be demonstrated by : — (a) Koch's method. Sections of the intestine, which must be well hardened in absolute alcohol, are left for twenty-four hours in a strong, watery solution of methylene-blue, or for a shorter time if the solution is warmed ; then treated in the usual way. (6) Babks' method. Sections, preferably from a recent case of cholera, an
. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. 366 INFECTIVE DISEASES. In sections of the intestine their presence may be demonstrated by : — (a) Koch's method. Sections of the intestine, which must be well hardened in absolute alcohol, are left for twenty-four hours in a strong, watery solution of methylene-blue, or for a shorter time if the solution is warmed ; then treated in the usual way. (6) Babks' method. Sections, preferably from a recent case of cholera, and made as soon as possible after death, are left for twenty-four hours in an aqueous solution of fuchsiue, then washed in distilled water, faintly acidulated with acetic acid, or in sublimate solution 1 in 1000, passed rapidly through alcohol, and finally treated in the usual way. Klein investigated cholera in India, and does not accept Koch's conclusions. With regard to the inoculation experiments, Klein believes that the living choleraic comma-bacilli, even if introduced in large numbers into the small intestine, are quite innocuous, but capable of great multipliqation if the intestine is previously, from some cause or another, diseased; the chemical products of the comma-bacilH then act as poisons analogous to the ptomaines obtained from other putrefactive bacteria. The observations made by Roy, Brown, and Sherrington, in Spain, tended to confirm Koch's views. Comma-bacilh were found to be present, in some cases, in enormous numbers, and the frequency of their occurrence led these observers to believe that they must bear some relation to the disease. At the same time, as they failed to find them in all cases, they regarded the existence of a causal relation as not proven. They failed to find the Naples bacterium, or a small, straight bacillus noted by Klein; and they drew attention to certain Tig. Organisms peculiar mycehum-hke threads WITH OTHKK BaCTERIA
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Keywords: ., bookcentury1800, bookdecade1890, bookpublisherphila, bookyear1897
