Practical physiological chemistry; a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . d absolute alcohol and pure dry ether.^Bloor: Jour. Biol. C/tem., 23, 317, Greenwald: Jour. Biol. Cliem., 21, 29, : Jour. Biol. Chem., 22, 133, 1915, 23, 317, and Egerer: J. Am. Chem. Soc, 37, 2373, and Miller: Jour. Biol. Chem., 18, 215, other nephelometric methods see Chapters XVII and • For Absorption Spectra see Plates I and II. ^ Stokes reagent is a solution containing 2 per cent ferrous su
Practical physiological chemistry; a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . d absolute alcohol and pure dry ether.^Bloor: Jour. Biol. C/tem., 23, 317, Greenwald: Jour. Biol. Cliem., 21, 29, : Jour. Biol. Chem., 22, 133, 1915, 23, 317, and Egerer: J. Am. Chem. Soc, 37, 2373, and Miller: Jour. Biol. Chem., 18, 215, other nephelometric methods see Chapters XVII and • For Absorption Spectra see Plates I and II. ^ Stokes reagent is a solution containing 2 per cent ferrous sulphate and 3 per cent tar-taric acid. When needed for use a small amount should be placed in a test-tube and ammo-nium hydroxide added until the precipitate which forms on the first addition of the hydrox-ide has entirely dissolved. This produces ammonium ferrotartrate which is a reducing agent. BLOOD ANALYSIS 297 a bright red to violet-red. The oxyhemoglobin has been reduced through theaction of Stokes reagent and hemoglobin (so-called reduced hemoglobin) hasbeen formed. This has been brought about by the removal of some of the loosely. Fig. 87.—AxouLAR-visioN Spectroscope Arranged for .\bsorptiox Analysis. combined oxygen from the oxyhemoglobin. Examine this hemoglobin spectro-scopically. Note that in place of the two absorption bands of oxyhemoglobin wenow have a single broad band lying almost entirely between D and E. This isthe typical spectrum of hemoglobin. If the solution showing this spectrum be
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