. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 3. A. Salt pond scum community dominated by sulftde oxidizing bacteria to the west of stake marked 4 on inset of Figure 1. B. Salina Bido, Matanzas Cuba, site of collection of the Cuba strain of Paratetramitusjugosus. Mangrove trees form the barrier between the mats and the open ocean. C. Microco- leus mat from Salina Bido, hand sample. With the help of two Earthwatch teams (1983) the La- guna Figueroa sites were extensively surveyed and marked with a permanent stake ("metal surveyor's stake," Fig. 1). Woode
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 3. A. Salt pond scum community dominated by sulftde oxidizing bacteria to the west of stake marked 4 on inset of Figure 1. B. Salina Bido, Matanzas Cuba, site of collection of the Cuba strain of Paratetramitusjugosus. Mangrove trees form the barrier between the mats and the open ocean. C. Microco- leus mat from Salina Bido, hand sample. With the help of two Earthwatch teams (1983) the La- guna Figueroa sites were extensively surveyed and marked with a permanent stake ("metal surveyor's stake," Fig. 1). Wooden stakes were placed as indicated by the open circles, including three reference stakes ("stakes A, B, C," Fig. 1). Sediment samples from the five sites were placed on petri plates to enrich for P. jugosus in early May; the experiments were repeated six weeks later in June 1983. Collections involving samples from the five different sediment types at Pentapus were twice made again in March and October 1988. Agar plates were prepared with two kinds of thinly poured sterile enrichment media: "modified-K" and "manganese-acetate" (MnAc) (Margulis el a/., 1980; Read et al, 1983). These plates were taken to the field study site where a sample about 1 mm3 of each of the five sediment types was placed directly at the center of both kinds of sterile plates. Immediately after samples were in place, they were cov- ered with approximately 1 ml of sterile distilled water to suspend the organisms and initiate reproduction and per- haps excystment. When vigorous growth was evident, plates were monitored and scored for the presence of cysts and amoebae. These were subcultured as needed onto fresh medium by streaking with a sterile platinum loop. The organisms were routinely grown at room temper- ature on either modified K or MnAc media, both of which contain half-concentrated seawater. The food source for P. jugosus is a gram-positive, flagellated, facul- tatively
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
