. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 662 RICHARD B. YULES C. Electrophysiology All recordings were made from the PDO nerve fibers in the meropodite (see Figure 2). The propodite and carpodite were immobilized on the side of a Petri dish in a vertical position with modeling clay (F), the dactylopodite remain- ing above the clay and thereby moveable, and the meropodite remaining below the clay. The exoskeleton of the meropodite and the two apodemes and their attached muscles were removed, allowing the nerve bundle to float free in Pantin's (1934) crustacean Ringe
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 662 RICHARD B. YULES C. Electrophysiology All recordings were made from the PDO nerve fibers in the meropodite (see Figure 2). The propodite and carpodite were immobilized on the side of a Petri dish in a vertical position with modeling clay (F), the dactylopodite remain- ing above the clay and thereby moveable, and the meropodite remaining below the clay. The exoskeleton of the meropodite and the two apodemes and their attached muscles were removed, allowing the nerve bundle to float free in Pantin's (1934) crustacean Ringer's solution (R). The nerves were then teased apart with sharpened tungsten needles and lifted individually or in small bundles into the air on a platinum wire electrode (E). The reference electrode was a platinum x UJ 20- 15- 10- K TIME OF MOLT O PDO TEST. 10 —I— 15 —i— 20 i 25 —i— 35 DAYS AFTER ES ABLATION FIGURE 1. Proecdysis in eyestalkless (ES) Sesarma, as determined by limb regeneration (R; solid black circles). The starred circles represent the points at which a leg was removed to provide a PDO for testing; see Table I. Note that A-E represent different animals. wire in the solution. Stimulating the PDO by moving the dactylopodite revealed a responding nerve bundle, which in preliminary experiments had been visually traced from the meropodite back to the PDO and which with methylene blue stain- ing at the site of the organ was shown to contain nerve fibers from all of the PDO receptors. Stimulating the PDO revealed no other responding nerve bundles. In some experiments fibers running with the PDO group, but not coming from the PDO, were found to fire in proecdysis crabs with R values above 10: these were separated from the PDO group. The preparation was iso- lated from vibration by mounting it on a heavy steel plate (V) resting on sawdust. The signal was amplified (Tektronix 122 preamplifier; AMP), displayed on an oscilloscope, and recorded on tape. The tape
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
