. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 4. (a) Ultrastructure of the surface of an intact unfertilized egg. A section stained with phosphotungustic acid (PTA). The first layer (1) of the chorion was stained intensively. (2, 3, 4) The second, third, and fourth layer of the chorion, respectively. > (b) Ultrastruc- ture of the isolated chorion. A section stained with PTA. The outer surface (arrow), which was morphologically similar to the third layer, was stained. (c) Sperm binding to the isolated chorions in artificial ordinary seawater. T
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 4. (a) Ultrastructure of the surface of an intact unfertilized egg. A section stained with phosphotungustic acid (PTA). The first layer (1) of the chorion was stained intensively. (2, 3, 4) The second, third, and fourth layer of the chorion, respectively. > (b) Ultrastruc- ture of the isolated chorion. A section stained with PTA. The outer surface (arrow), which was morphologically similar to the third layer, was stained. (c) Sperm binding to the isolated chorions in artificial ordinary seawater. Two minutes after insemination. X140. Sperm were bound to the isolated chorion in both or- dinary and Ca-free seawater, and the bindings of sperm were kept for a long time (Fig. 4c). Because the isolated Table I Percentage of occurrence of acrosome reaction in \pcnn hound to isolated chorion in presence or absence of Co?* Percentage of acrosome reaction Expt. No. Ordinary seawater Ca-free seawater 1 2 0 3 4 0 Isolated chonons were fixed 1-10 min after insemination. Occur- rence of acrosome reaction was checked by phase contrast microscopy in 200-300 spermatozoa on 3-6 chorions. chorion was transparent, the acrosome reaction of the attached sperm could be checked by phase-contrast mi- croscopy. In ordinary seawater, many sperm underwent the acrosome reaction (Table I, Fig. 5). These sperm were also examined by electron microscopy. The lobular acro- somal process adhered to the outer surface of the chorion and did not penetrate it (Fig. 6a). In Ca-free seawater, the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, C
Size: 1827px × 1368px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
