. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 252 A. E. NAVEZ AND B. KROPP chamber at ° C. The cutting is done with the aid of a double razor blade device, previously wiped through a block of paraffin. Coleoptiles of Avena, of an average length of 30 to 35 mm., were decapitated by means of a small knife with a sharp V-shaped notch (also paraffined). Portions of tips to 2 mm. long were removed. The root tips of Lupinus were removed to a similar length. The coleoptiles used were of seeds grown in sawdust in small glass vessels. The coleoptiles passed through a glas
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 252 A. E. NAVEZ AND B. KROPP chamber at ° C. The cutting is done with the aid of a double razor blade device, previously wiped through a block of paraffin. Coleoptiles of Avena, of an average length of 30 to 35 mm., were decapitated by means of a small knife with a sharp V-shaped notch (also paraffined). Portions of tips to 2 mm. long were removed. The root tips of Lupinus were removed to a similar length. The coleoptiles used were of seeds grown in sawdust in small glass vessels. The coleoptiles passed through a glass collar fixed in a sheet of hard rubber which was attached to the glass vessel; this avoids accidental bending or displacement of the coleoptiles during manipulation. Measurement of the coleoptiles is car- ried on while they are in a glass trough lined with filter paper; one side c/o o: t DQ a. o z bJ. TIME (I UNIT = 60 MIN.) FIG. 1. Elongation of decapitated Avena coleoptiles under the influence of Palccmonetes eye-stalk extract. In this and subsequent figures arrow indicates point at which the extract-imbibed agar block was applied. of the glass trough is hinged, which permits removal of water of gutta- tion. The roots, of an average length of 25 to 30 mm. when used, were grown in vials 100 X 25 mm., lined with moist filter paper except for a vertical ;' window "; the seeds were held in paraffined paper funnels attached to the vials by paraffin blocks. Decapitation and subsequent manipulation of the seedlings were car- ried out in dim red light. The agar blocks were placed at one side of the decapitated coleoptile, beside the projecting first leaf, with the help of a glass needle. The drop of water of guttation which appears on the uncovered half of the end of the coleoptile when the block is in place was removed at intervals by means of filter-paper strips; this prevented the displacement of the agar Please note that these images are extracted from scanned pag
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
