. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 346 WALTER G. WHITFORD AND VICTOR H. HUTCHISON film which funned on the surface and which would have resulted in reduced ab- sorption of carbon dioxide. Oxygen injected into the chambers by the syringes compensated for oxygen consumed by the animal. Oxygen consumption was read directly from the calibrated syringes. At the end of a set of experiments, the beakers of barium hydroxide were re- moved from the chambers and titrated with standardized 1 N sulfuric acid to determine the quantity of carbon dioxide produced. The beake
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 346 WALTER G. WHITFORD AND VICTOR H. HUTCHISON film which funned on the surface and which would have resulted in reduced ab- sorption of carbon dioxide. Oxygen injected into the chambers by the syringes compensated for oxygen consumed by the animal. Oxygen consumption was read directly from the calibrated syringes. At the end of a set of experiments, the beakers of barium hydroxide were re- moved from the chambers and titrated with standardized 1 N sulfuric acid to determine the quantity of carbon dioxide produced. The beakers of barium hy- droxide in the thermobarometers served as controls, since each beaker of barium hy- droxide absorbed carbon dioxide at the same rate both prior to the experiment and during the time required for titration. To determine the actual amounts of carbon dioxide released by the animal, the amount of carbon dioxide absorbed in the 02 TR TB. WJ-- B S ^ 10 cm FIGURE 2. Apparatus used to measure simultaneously pulmonary and cutaneous respiration in amphibians. AM, animal mask; B, barium hydroxide solution in beaker; C, cooling coil; H, heating coil; M, manometer; Mg, magnetic stirring bar; O2, oxygen syringe; TB, thermo- barometer chamber ; TR, temperature regulator ; S, stirrer; WJ, water jacket. thermobarometers was subtracted from the amounts of carbon dioxide absorbed in the pulmonary and cutaneous chambers. The few animals that struggled against their bonds during the first hour of the experiment produced high oxygen consumption values. The values obtained for these hours were not included in the calculations of mean oxygen consumption, but the carbon dioxide produced had to be included in the determination of respiratory quotients, since the barium hydroxide could not be removed for titration after each hour. Measurements of total oxygen consumption were made as controls. Differences in oxygen consumption between masked and unmasked animals were not statisti- cally
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
