. A text-book of bacteriology. Bacteriology. 454 BACTERIA IX DIPHTHERIA. It grows readily in nutrient gelatin having a sliglitlj- alkaline reac- tion, in nutrient agar, glycerin-agar, or in alkaline bouillon, but the most favorable medium appears to be that first recommended by Loffler—-viz., a mixture of three -^^w^^/ff. -Colonies of Bacillus dlphtherpse in nutrient agar, end ot twenty-four hours. X 10. CFrunkel and PfelfEer.) "r> parts of blood serum with one part of bouillon, containing one per cent of peptone, one per cent of grape sugar, and per cent of sodium chloride. This m
. A text-book of bacteriology. Bacteriology. 454 BACTERIA IX DIPHTHERIA. It grows readily in nutrient gelatin having a sliglitlj- alkaline reac- tion, in nutrient agar, glycerin-agar, or in alkaline bouillon, but the most favorable medium appears to be that first recommended by Loffler—-viz., a mixture of three -^^w^^/ff. -Colonies of Bacillus dlphtherpse in nutrient agar, end ot twenty-four hours. X 10. CFrunkel and PfelfEer.) "r> parts of blood serum with one part of bouillon, containing one per cent of peptone, one per cent of grape sugar, and per cent of sodium chloride. This mixture is steril- ized and solidified at a low tem- perature, as is ugual with blood serum. Upon this the develop- ment is so rapid in the incubating oven that, at the end of twentj'- four hours, the large, round, ele- vated colonies, of a graj'ish-white color and moist appearance, may be easily recognized, while other associated bacteria will, as a rule, not yet have developed colonies large enough to interfere with the recognition of these. Upon nutrient agar plates the deep-lying colonies, when magni- fied about eighty diameters, appear as round or oval, coarsely granu- lar discs, with rather ill-defined margins, or, when several colonies are in juxtaposition, as figures of irregular form. The superficial col- onies are grayish-yellow in color, have an irregular, not well-defined outline and a rough, almost reticulated surface. The growth upon glycerin-agar is very similar. The first inoculations in a plain nu- trient agar tube often give a comparatively feeble growth, which be- comes more abundant in subsequent inoculations in the same medium. In stick cultures in glycerin—or plain—agar, growth occurs to the bottom of the line of inoculation, and also upon the surface, but is not at all characteristic. The same may be said with reference to cultures in nutrient gelatin. Plate cultures in this medium contain- ing fifteen per cent of gelatin, at 24° C, give rather smal
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