. The Biological bulletin. Biology; Zoology; Marine biology. 212 ALBERT TYLER, BETTY S. TYLER AND JORAM PIATIGORSKY Hultin, 1960; Hultin, 1961) to take place. While an understanding of the basis for the difference would be of interest, this would not alter the main conclusion to be drawn from the experiments, which is that the incorporation of amino acid into protein by the unfertilized egg evidently represents protein synthesis. â UNFERTILIZED (5400 cpm/lO^eggs). " PUROMYCIN CONCENTRATION x 10" ,-4 MOLAR Figure 1. Action of puromycin on incorporation of
. The Biological bulletin. Biology; Zoology; Marine biology. 212 ALBERT TYLER, BETTY S. TYLER AND JORAM PIATIGORSKY Hultin, 1960; Hultin, 1961) to take place. While an understanding of the basis for the difference would be of interest, this would not alter the main conclusion to be drawn from the experiments, which is that the incorporation of amino acid into protein by the unfertilized egg evidently represents protein synthesis. â UNFERTILIZED (5400 cpm/lO^eggs). " PUROMYCIN CONCENTRATION x 10" ,-4 MOLAR Figure 1. Action of puromycin on incorporation of C"-valine into protein by unfertilized and fertilized eggs of Lytechinus pictus. The eggs were incubated with the C"-valine for 60 minutes at 20° C, starting 30 minutes after addition of the puromycin, which occurred at 30 minutes after fertilization for the fertilized eggs. Each tube contained in ml. final volume, 10,000 unfertilized or 2000 fertilized eggs, puromycin at the indicated concentrations, and IXC. C'*-valine (sp. act. ). Each set of experiments was run in duplicate and the "error bars" are drawn between the two values. 3. Polysomes in unfertilised eggs In several investigations (Monroy and Tyler, 1963; Stafford et al., 1964; Malkin, Gross and Romanoff, 1964; Piatigorsky, 1966; Nemer and Infante, 1967; Stavy and Gross, 1967) it has been noted that the polysome region of sucrose density gradients of homogenates of unfertilized eggs shows RNA-containing material which, however, is largely insensitive to RNase. The low incorporating activity of the unfertilized egg has impeded attempts to determine whether or not some fraction of this material represents polysomes actively engaged in protein synthesis. However, this difficulty has now been largely overcome by the use of non-competing mixtures of labeled amino acids (Tyler, Piatigorsky and Ozaki, 1966). The results of two sets of experiments with unfertilized and fertilized
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