. The Journal of laboratory and clinical medicine . NG a paper on the natural immunity of the rabbit for atropinewhich has appeared in an abbreviated form in the Arch. Neerl. de Physi-ologie (T V, 3 me Livr., p. 380, 1921), I had to examine all alkaloid reagentswith regard to their applicability for the quantitative determination of atro-pine, because I found that no one had done this before me. As I think thatthis preliminary work, which cost me much time, may be of some use to laterstudents of this subject, I publish herewith its results. The determination of small quantities of the alkaloid
. The Journal of laboratory and clinical medicine . NG a paper on the natural immunity of the rabbit for atropinewhich has appeared in an abbreviated form in the Arch. Neerl. de Physi-ologie (T V, 3 me Livr., p. 380, 1921), I had to examine all alkaloid reagentswith regard to their applicability for the quantitative determination of atro-pine, because I found that no one had done this before me. As I think thatthis preliminary work, which cost me much time, may be of some use to laterstudents of this subject, I publish herewith its results. The determination of small quantities of the alkaloid in blood serum canimmediately be divided into two parts: (1) The isolation of the alkaloid outof the complicated chemical mixtvire which we call serum. (2) The deter-mination of atropine in the watery solution thus obtained. I shall treatthese two parts separately. 1. ISOLATION OF THE ALKALOID In forensic chemistry such a case would demand the method of Stass-Otto, which has been accurately described in the Lehrbuch de? gerichtlichen HuBBER BANl). TO /IRPUMP Fig. 1.—Apparatus for microfiltration. The liquid passes through two pieces of filter paperkept tightly between the two pieces of the apparatus by means of rubber bands. Medizin of Baumert. Without comparing it in detail with the other exist-ing methods, as for instance that of Kippenberger—the bibliography showedme that one of them is as good and bad as the other—I immediately tried to *From the Physiological Laboratory of the Free University in Amsterdam, J. J. Buytendyk. 280 Dir. Prof. Dr. ATROPINE IN BLOOD SERUM 281 adapt this method to microchemical analysis. Using small test tubes, con-taining about 15 , with a thick wall, so that they could be used in thecentrifuge, the amount of atropine was determined in one of serum, con-taining various amounts of atropine dissolved in one of water. Immedi-ately after they had been taken out of the incubator, they were filled withabsolute alcohol, a
Size: 925px × 2701px
Photo credit: © Reading Room 2020 / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1910, booksubject, booksubjectmedicine
