. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 446 D. M. C. MUTTON AND SMITH 3 -i - - No catalase. i - PMA concentrations ( Mg ml"1) - - -C] 1 mi"1 catalase No catalase ml"1 catalase -•- 8 >;g ml"1 catalase LPS concentration (tig mj"1) Figure 3. Effect of different phorbol mynstate acetate (PMA). lipopolysaecharide (LPS), and catalase concentrations on the reduction of ferricytochrome c by isolated amoebocytes from Actinia equina in vitro. Twenty-five microliters of wa
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 446 D. M. C. MUTTON AND SMITH 3 -i - - No catalase. i - PMA concentrations ( Mg ml"1) - - -C] 1 mi"1 catalase No catalase ml"1 catalase -•- 8 >;g ml"1 catalase LPS concentration (tig mj"1) Figure 3. Effect of different phorbol mynstate acetate (PMA). lipopolysaecharide (LPS), and catalase concentrations on the reduction of ferricytochrome c by isolated amoebocytes from Actinia equina in vitro. Twenty-five microliters of washed amoebocyles in ocean salts (OS; final concentration • 104ml ')was incubated with 25 M' of PMA (a) or LPS (b) (both at final concentrations of I, 4, or 8 Mg ml'), 25 n\ of catalase (final concentrations 0, 1. 4, or 8 Mg ml '), 25 M' of OS and 50/jl of ferricytochrome c (final concentration 64 pAf). For controls, the PMA or LPS and catalase was replaced by 50 ^1 of OS and the absorbance of each well was measured at 550 nm after 5 min. Values given are means ± SE; n = 5. stead, the cells appear to release one or more bactericidal factors upon exposure to Tween 20. Given that the SI for both experimental and control amoebocytes was below 100 (P < ) (Fig. 6), at least one of these factors is a lysin. Antibacterial activity ofALS That the antibacterial activity by A. equina amoebo- cytes was due to one or more soluble, rather than mem- brane-bound, factors was confirmed by the results of the experiments with ALS. Experimental (ALS-incubated) bacteria showed significantly poorer growth than con- trol P. immobilis incubated in OS supplemented with MB (P < after 18-h incubation at 20°C) (Fig. 7). Unfortunately, this assay failed to confirm that the antibacterial effect of A. equina ALS involves lysis, pos- sibly because the rapid growth of surviving bacteria would have masked any reduction in bacterial turbidity. Please note that these images are extrac
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
