. Dourine in Canada, 1904-1920 [microform] : history, research and suppression. Dourine; Dourine; Chevaux; Horses. 28 can actually bo used with perfect safety provided the serum is correctly inativatcd But it is unnece»8nry to use more than 0-2 , for that amount of ^crum fif a dourino tested-- the first and last to contain 0-2 of undiluted serum, the larfrest amount used in the tc^t, the last tube heinfr the scrum control without antifren, the interveninp tubes to contair gradually decreasing doses of serum. Enough salt solution is then added to make up to 1-0 in each tube, then t
. Dourine in Canada, 1904-1920 [microform] : history, research and suppression. Dourine; Dourine; Chevaux; Horses. 28 can actually bo used with perfect safety provided the serum is correctly inativatcd But it is unnece»8nry to use more than 0-2 , for that amount of ^crum fif a dourino tested-- the first and last to contain 0-2 of undiluted serum, the larfrest amount used in the tc^t, the last tube heinfr the scrum control without antifren, the interveninp tubes to contair gradually decreasing doses of serum. Enough salt solution is then added to make up to 1-0 in each tube, then tiie antiscn and complement in amounts previously determined by careful titration, and fiiuilly, afto- incubation for seventy minutes, haemolytic serum and red cells—as in a diagnostic test. An experiment of this kind is given l)clow, the titres of seven sera from ditfi'renl horses in different iihascs of the disease being determined. ;'rimcnt for dilirmininff (lip Atii'lodii content of Vinivhu^ Srra Ini tlf Complemrnl Fixation Method. Dose of iniicf ivatfMi ituurino sorum ('(jiiiplpinent (ixation reactions with ilourini' mtuiii NO. .-•. .\o. 7 + + + + + + + + moans complete fix"*ion of romplemnt-absolutely no tr:ii(.' of Hed lella morp or elumpeil. \ very stronK positi\f reiictitm. -i—f- + is also u strong? positive reaction, witli just a trace or tinp;e of haemolysis. -f + is a ratlier weak positive, indicating partial fixation -about one-half the red cells iiaemulysed, + , a very weak or faint positive—slight fixation, with more than oni^-half the re<l cells liaemolyaed. —, a negative reaction. Complete haemolysis of red cells. The smallest dose of serum which comhiiies witli antigen to cause complete fixation ( +-+-f 4-) indicates the value of one antibody unit, iind from this may bo calculated the number of units in the or maximum dose and the value of a douriue scrum in antibody content. rif^m^^^wtii^^i^^^^m^bs
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Keywords: ., bookcentury1900, bookdecade1920, booksubjecthorses, bookyear1920
