. American journal of pharmacy. tary extract are meas-ured out with a narrow pipette into a small, conical, glass cup anddiluted with enough Lockes solution to make about i Cc. Fromthis glass the dilution is taken up with a Record syringe havinga long needle, and introduced into the chamber, the glass andsyringe being afterward rinsed with another i Cc. of Lockes so-lution. The beta-imidazolylethylamine hydrochloride was supplied bythe Hoffman-La Roche Co. .05 gram of this substance takenfrom the i gram vials as supplied, without desiccating (being appar-ently dry), is placed in a sterile 500
. American journal of pharmacy. tary extract are meas-ured out with a narrow pipette into a small, conical, glass cup anddiluted with enough Lockes solution to make about i Cc. Fromthis glass the dilution is taken up with a Record syringe havinga long needle, and introduced into the chamber, the glass andsyringe being afterward rinsed with another i Cc. of Lockes so-lution. The beta-imidazolylethylamine hydrochloride was supplied bythe Hoffman-La Roche Co. .05 gram of this substance takenfrom the i gram vials as supplied, without desiccating (being appar-ently dry), is placed in a sterile 500 Cc. volumetric flask and dis-solved in freshly boiled and cooled distilled .water containing a smallamount of acetoform and made up to mark. This i : dilutionIS sealed in ampoules and kept in a refrigerator in the dark. Itseems to keep, thus, for many months without noticeable deteriora-tion. For use 10 Cc. are diluted to 100 Cc. in a volumetric flask. 200 U. S. p. Standard for Pituitary Extract. { Am. jour, i Of this I : 100,000 dilution .2, .25, .4, or .5 Cc. are added to the100 Cc. chamber containing the uterus when a dikition of i: 50,000,-000, 1:40,000,000, 1:25,000,000, or 1:20,000,000 respectively isdesired. ^°^May? fg^.r. ) ^^ ^- ^- Standard for Pituitary Extract. 201 Comparative Activity. During the past three years I have made over 500 kyniographictracings of the isolated uterus in testing pituitary glands, solutions,and finished commercial extracts made in this laboratory and alsoin others, and during this experience I have never found it necessaryto apply as low a dilution as i: 20,000 of any commercial extractwithin the age limit. Indeed, it is rare that I ever apply a i: 100,000dilution. The uteri on the average have required dilutions of from1: 160,000 to 1: 600,000, occasionally i: 800,000 to i: 1,000,000, andrarely i: 2,000,000. This fact by itself is not of much importance,since some uteri are extremely sensitive, but it is significant in c
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Keywords: ., bookcentury1800, bookidamerica, booksubjectpharmacy, bookyear1829
