. Contributions. Biology. THE GAS PRODUCTION OF BACILLUS COLL 75 mercury from passing over into the pentoxide tube. The gas is delivered into the tube B, which stands in a mercury trough. The tubes G and G are the Geissler tubes used for examining the residual gas spectroscopically. F is a McLeod gauge used to measure the vacuum. This gauge was subse- quently removed so as to leave less space to be exhausted, as it had served its purpose as soon as the efficiency of the apparatus was known. From the tube B the gas was trans- ferred to the gas burette for analysis. The bulb E was the. container
. Contributions. Biology. THE GAS PRODUCTION OF BACILLUS COLL 75 mercury from passing over into the pentoxide tube. The gas is delivered into the tube B, which stands in a mercury trough. The tubes G and G are the Geissler tubes used for examining the residual gas spectroscopically. F is a McLeod gauge used to measure the vacuum. This gauge was subse- quently removed so as to leave less space to be exhausted, as it had served its purpose as soon as the efficiency of the apparatus was known. From the tube B the gas was trans- ferred to the gas burette for analysis. The bulb E was the. container in which the organism was grown. Figure 2 is a more detailed drawing of this bulb. A and B are the stop- cocks and C a tube which contained a few cubic centimeters of culture fluid which were inoculated with the organism to be studied and later allowed to flow into the bulb to inocu- late it. The stopcocks were ground with jeweller's rouge previous to use. The whole apparatus was set up in a cellar room where the temperature was very uniform. The complete manipulation was as follows : The vacuum culture bulb was thoroughly cleaned with concentrated sul- phuric acid and rinsed until every trace of acid was removed. The stopcocks were wiped dry with filter paper, greased very lightly to lubricate. The lubricant used was composed of the following substances: Gutta-percha three parts, hard white paraffin one part, and enough pure heavy mineral oil to make the resulting mixture of the same consistency as ordinary lanolin. The bulb thus prepared is exhausted with. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Brown University. Biological Laboratory. Providence, R. I
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Keywords: ., bookcentury1800, bookdecade1890, bookpublishe, booksubjectbiology
