. American bee journal. Bee culture; Bees. 1920 AMERICAN BEE JOURNAL 227 served, the likelihood of robbing, swarming, absconding and accidental straying or drifting of bees is re- duced. In making the inoculations two methods were used. Those have been referred to as the direct and the in- direct methods, respectively. Follow- ing the direct method, a group of about 10 to 20 larva;, 2 to 3 days old, are fed thin sugar syrup to which has been added the contents of stomachs of sick larvse. This is done by put- ting a very small quantity of the con- taminated syrup directly with the food of the l
. American bee journal. Bee culture; Bees. 1920 AMERICAN BEE JOURNAL 227 served, the likelihood of robbing, swarming, absconding and accidental straying or drifting of bees is re- duced. In making the inoculations two methods were used. Those have been referred to as the direct and the in- direct methods, respectively. Follow- ing the direct method, a group of about 10 to 20 larva;, 2 to 3 days old, are fed thin sugar syrup to which has been added the contents of stomachs of sick larvse. This is done by put- ting a very small quantity of the con- taminated syrup directly with the food of the larva within the cell. For this purpose a small glass tube is used Care must be taken that too much syrup is not added in making the inoculations, as the larvae would be floated thereby. Care must be observed also that they are not disturbed mechanically by the tube. Disturbances of this kind are very likely to be followed by their re- moval by the adult bees. Within 3 days the inoculated larvae show marked symptoms of the disease, some of the sick or dead ones are re- moved by the end of the third day and most of them before the end of the fourth day. Only a few are found infected outside the arena of brood inoculated. The colony usually speedily recovers from the infection and no further evidence of the dis- ease is seen. By the indirect method the entire colony is inoculated through feeding it about one-third of a pint of sugar syrup to which the crushed bodies of from S to 10 larvae sick or dead of the disease have been added. The first symptoms of the disease are ob- served about 3 days after inoculation. The youngest larvae to show symp- toms are about 4 days old. After a single feeding the colony usually soon recovers from the disease. Other factors being equal, the recovery is more rapid when the amount of brood is small in proportion to the strength of the colony, when the flow of nectar is good, and when the bees are active. A stock of fresh disease material is needed
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Keywords: ., bo, bookcentury1800, bookdecade1860, booksubjectbees, bookyear1861
