. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 276 S. R. RODRIGUEZ ET AL. 100. 18 24 TIME (h) Figure 3. Mean percentage of early pre-competent larvae of Concholepas concholepas induced to sink by extracellular matrix (ECM) components and sulfated polysaccharides. (a) Mean percentage of C concholepas larvae induced to sink by ECM constituents. • = Fibronectin, O = gelatin. • = collagen, and D = control, (b) Mean percentage of O concholepas larvae induced to sink by sulfated poly- saccharides. • = Carrageenan, • = chondroitin sulfate, O = heparin. and D = control. 10 mM NH
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 276 S. R. RODRIGUEZ ET AL. 100. 18 24 TIME (h) Figure 3. Mean percentage of early pre-competent larvae of Concholepas concholepas induced to sink by extracellular matrix (ECM) components and sulfated polysaccharides. (a) Mean percentage of C concholepas larvae induced to sink by ECM constituents. • = Fibronectin, O = gelatin. • = collagen, and D = control, (b) Mean percentage of O concholepas larvae induced to sink by sulfated poly- saccharides. • = Carrageenan, • = chondroitin sulfate, O = heparin. and D = control. 10 mM NH4C1 were washed and placed in normal fresh seawater to see if they would recover. Three hours later, all larvae were observed swimming normally (data not shown). Chemotaxis to microhial films. Microbial films are well known as settlement inducers for a number of benthic marine invertebrates. We studied the attraction responses of pre-metamorphic C. concholepas larvae exposed to several such films. In the chemotaxis experiment in which glass plates were coated with different films, the larvae responded positively to P. marimis. multispecific. diatom, and bacterial films after 25 min of incubation: rates of attraction were20%. 30%, 20%, and 20% respectively (Fig. 5a). At that time, no significant difference was observed among the different films (G test with 3 df). However, after 60 min of incubation, the respective rates of larval attraction increased to 40%., 60%, 60%. and 30%, and a significant preference was observed for the multispecific and the diatom films compared to the P. marimis and bacterial films (P < , G test with 1 df) (Fig. 5a). When C. concholepas shells were used as substrata, a pos- itive response of larvae to P. marimis, multispecific, and diatoms films was observed after 25 min of incubation; rates of attraction were 30%, 40%, and 10%, respectively (Fig. 5b). At that time, the attraction to the multispecific and P. marimis films was significat
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
