. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 6. Effects of nocodazole on ooplasmic segregation. (A) Control egg, tn = A large blastodisc has formed at the animal pole, and oil droplets have formed a cap over the vegetal hemisphere. (B) Egg treated with nM nocodazole. tn = The blastodisc is smaller than in the control egg, and most of the oil droplets, instead of moving toward the vegetal pole, have floated to the top of the egg ( toward the viewer, whose perspective is from above the egg) and coalesced into one large droplet there. Scale ba
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 6. Effects of nocodazole on ooplasmic segregation. (A) Control egg, tn = A large blastodisc has formed at the animal pole, and oil droplets have formed a cap over the vegetal hemisphere. (B) Egg treated with nM nocodazole. tn = The blastodisc is smaller than in the control egg, and most of the oil droplets, instead of moving toward the vegetal pole, have floated to the top of the egg ( toward the viewer, whose perspective is from above the egg) and coalesced into one large droplet there. Scale bar, 250 Mm. situation in control eggs, in which the male and female pronuclei were within 5 ^m of each other, the male and female pronuclei in colchicine-treated eggs were far apart ( ± urn; X ± , n = 8; Fig. 8E). Discussion All three microtubule poisons used in this study—col- chicine, colcemid. and nocodazole—affected the move- « o m ^ a> c JC u 175 150 125 100 75 50 25. 00 06 Proportion of Time to First Cleavage Figure 7. Effect of microtubule poisons on the growth of the blas- todisc. The thickness of the blastodisc along the animal-vegetal axis was measured in untreated eggs (— • —. four eggs) and in eggs treated with 100 iiM fj-lumicolchicine (— D —, three eggs), 100 pM colchicine (— • —, four eggs), nM colcemid (— O —. three eggs), or tiM nocodazole (— A —. five eggs). Shown are the mean values; over all the treatments and times, the standard deviation averaged 12% of the mean. Colcemid, colchicine. and nocodazole all slowed the growth of the blas- todisc. while eggs treated with £J-lulmicolchicine behaved as untreated eggs. The volume of the blastodisc in control eggs_and poisoned eggs at tn = was ± nl and ± nl (X ± SD), respectively. ment of ooplasmic inclusions during segregation in the medaka egg. That the poisons acted specifically as micro- tubule poisons is
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
