. Ciba Foundation colloquia on ageing. Old age; Aging; Animals -- growth & development. Human Red Cell Instability and Senescence 219 but depends upon the previous treatment of the cells. It increases with the length of time the cells have been stored in vitro and decreases with the number of times they have been washed. The effect of repeated suspensions in 0-16 M-NaCl is shown in Fig. 1. Even after 20 washes only 6 per cent of the cells have haemolysed, and the proportion haemolysing was con- stant for each washing. During the first 7 washes, however,. 10 20 30 40 50 TIME (MINUTES) 60 Fi
. Ciba Foundation colloquia on ageing. Old age; Aging; Animals -- growth & development. Human Red Cell Instability and Senescence 219 but depends upon the previous treatment of the cells. It increases with the length of time the cells have been stored in vitro and decreases with the number of times they have been washed. The effect of repeated suspensions in 0-16 M-NaCl is shown in Fig. 1. Even after 20 washes only 6 per cent of the cells have haemolysed, and the proportion haemolysing was con- stant for each washing. During the first 7 washes, however,. 10 20 30 40 50 TIME (MINUTES) 60 Fig. 2. The rate of removal of components from the red cell by exposure to alumina. Cells suspended in 0 16 M-NaCl were exposed to powdered alumina (500 mg. A1203 per ml. of packed cells) at 37° 0 Dry weight of lipids; + Lipoprotein; (J Cholesterol; X Phospholipid; O Haemoglobin. a considerable amount of the cell membrane was dispersed in the medium and shrinkage of the cells took place. There- after the losses of cell components proceeded at a rate equi- valent to the loss of cells by haemolysis. Figs. 2 and 3 show that the effects of exposure to alumina are closely similar to those of repeated washing. The haemo- lysis of the cells is directly related to the time of exposure and concentration of alumina. The loss of lipids, however, bears a direct relationship to the time and intensity of exposure only. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ciba Foundation; Wolstenholme, G. E. W. (Gordon Ethelbert Ward). Boston, Little, Brown
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