. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PERICARDIAL ORGAN ACTIVE PEPTIDES 11 was taken up in dilute NH4OH and applied to the center of the paper. A paper with lysine, glycine and glutamic acid applied at the origin was run simultaneously with the hydrolysate as a standard. RESULTS [nactivation of pericardial organ active suhstance was accomplished by incu- hating extracts made in Ho mar us perfusion fluid (pH ) with crystalline trypsin or chymotrypsin (approximately 10 ^ extract) for one hour at 37° C. The enzymes alone had little, if any, effect on the h
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PERICARDIAL ORGAN ACTIVE PEPTIDES 11 was taken up in dilute NH4OH and applied to the center of the paper. A paper with lysine, glycine and glutamic acid applied at the origin was run simultaneously with the hydrolysate as a standard. RESULTS [nactivation of pericardial organ active suhstance was accomplished by incu- hating extracts made in Ho mar us perfusion fluid (pH ) with crystalline trypsin or chymotrypsin (approximately 10 ^ extract) for one hour at 37° C. The enzymes alone had little, if any, effect on the heart. Heated enzyme incuhated with pericardial organ extract did not affect the activity. These results confirm those obtained by Maynard and Welsh (1959). Trypsin was used periodically to in- activate extracts and eluates from paper electrophoresis and chromatography. This gave assurance that the activity was due to pericardial organ cardio-excitor substance. A typical assay of a pericardial organ extract after electrophoresis is shown in Figure 1. As was true in most assays, the activity was spread over several centi- meters with a major peak at one fraction. Where activity was found to be equally distributed between fractions, it is assumed that the fraction boundary line cut the activity peak in two. The data in Figure 2 demonstrate that the isoelectric point of the active material is near pH 3, and an increase in pH above this point causes a corresponding increase. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
