. Pharmaceutical bacteriology, with special reference to disinfection and sterilization. Bacteriology. BACTERIOLOGICAL TECHNIC. 55 Also make streak inoculation in tube slants by simply passing the infected platinum needle over the middle of the tube slant surface, from lower end toward the top, observing the instructions regarding the cotton plug and needle sterilization, with each tube inoculation. Number the tubes seri- ally, and in a special notebook make entry of all desirable data pertaining to each inoculation, making such entries under each tube number. Place tubes vertically in a suita
. Pharmaceutical bacteriology, with special reference to disinfection and sterilization. Bacteriology. BACTERIOLOGICAL TECHNIC. 55 Also make streak inoculation in tube slants by simply passing the infected platinum needle over the middle of the tube slant surface, from lower end toward the top, observing the instructions regarding the cotton plug and needle sterilization, with each tube inoculation. Number the tubes seri- ally, and in a special notebook make entry of all desirable data pertaining to each inoculation, making such entries under each tube number. Place tubes vertically in a suitable holder, as tumbler, beaker, wire basket, etc., and set aside in incubator or in some container to which you alone have access. In warm weather the first bacterial growths may appear at the end of thirty-six hours. In cold or cool weather nothing may appear for two, three, and even four to five days. Note the nature of the bacterial growth in a deep stab inoculation and in the streak inoculation, as to. Fig. 32.—Making an Esmarch roll-tube culture. A lump of ice is placed in a dish and the inoculated tube is placed horizontally in a groove in the ice and revolved undl the medium is well set. The groove may be made with test-tube full of hot water. {Williams.) a. Growth—scanty, moderate, abundant, slow, rapid. b. Form of growth—outlme clearly defined, spreading, rugose, beaded, etc. c. As to surface'—Qa,t, raised, concave, convex. d. CoZo;-—translucent, glistening, waxy, transparent, opaque, light, chalky white, grayish-white, dark red, green, blue, yellow, lemon color, purple, etc. e. Odor—comparative description. f. Consistency—viscid, slimy, stringy, membranous, friable or brittle, dry, watery, etc. g. Changes in medium—gdaXin liquefied, gelatin not liquefied; colored, as grayed, browned, reddened, blued, etc. In case indicators are used, the possible color changes should be Please note that these images are extracted from scanned page images that m
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