. The Biological bulletin. Biology; Zoology; Marine biology. Vol. XXVIII. May, 1915. No. 5. BIOLOGICAL BULLETIN ON THE RHYTHMICAL SUSCEPTIBILITY OF DE- VELOPING SEA URCHIN EGGS TO /«9' HYPERTONIC SEA WATER. ARTHUR RUSSELL MOORE. (From the Biological Laboratory of Bryn Mawr College and the Marine Biological Laboratory at Woods Hole.) In a recent communication M. Herlant^ attempting an analysis of Loeb's method of artificial parthenogenesis concludes that (i) the fatty acid treatment gives rise to the rhythmical activity of the centrosome but never to normal divisions of the egg; (2) the treatme


. The Biological bulletin. Biology; Zoology; Marine biology. Vol. XXVIII. May, 1915. No. 5. BIOLOGICAL BULLETIN ON THE RHYTHMICAL SUSCEPTIBILITY OF DE- VELOPING SEA URCHIN EGGS TO /«9' HYPERTONIC SEA WATER. ARTHUR RUSSELL MOORE. (From the Biological Laboratory of Bryn Mawr College and the Marine Biological Laboratory at Woods Hole.) In a recent communication M. Herlant^ attempting an analysis of Loeb's method of artificial parthenogenesis concludes that (i) the fatty acid treatment gives rise to the rhythmical activity of the centrosome but never to normal divisions of the egg; (2) the treatment of the eggs with hypertonic sea water causes the formation of accessory asters and is necessary to complete the causes for normal division; (3) the optimum results are obtained by applying the hypertonic solutions at certain intervals after fatty acid treatment, viz., 30 and 70 minutes and possibly 115-120 minutes, while with 40-50 and 95-100 minute intervals marked minima are shown. As to the first generalization, Herlant ignores the fact that in Strongylocentrotus and Arhacia the fatty acid treatment alone may cause normal segmentation. If the eggs of S. purpuratus are kept at a low temperature (5°-io°) after acid treatment alone, they divide regularly and may reach the morula stage.^ In Arhacia eggs, normal segmentation may take place after acid treatment without subsequent treatment with the hyper- tonic solution, but does not as a rule proceed beyond the two- cell stage. On the other hand hypertonic treatment alone may cause Arhacia eggs to segment and develop into swimming larvse, while it brings about only early segmentation stages in the eggs of Strongylocentrotus. 1 M. Herlant, Comptes Rendus de I'Academie, T. 158, p. 1531. ^ J. Loeb, "Artificial Parthenogenesis and Fertilization," p. 76. 253. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these il


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