. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 264 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS. Figure 1. (Al Mitotic spindle in Xenupus egg extract. Fluorescent speckles in the microtubules were visualized by adding \-rliodamine-labeled tithulin (~ l'7r "I total tnhulin in extract! anil using the CSU-IO confocal unit as described in the text. Arrows point to free microtubules extending from the spindle. iBl Kymograph showing the dynamics of fluorescent speckle flux. The kymograph is made up by displaying, on the horizontal axis, the fluorescence distribution a
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 264 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS. Figure 1. (Al Mitotic spindle in Xenupus egg extract. Fluorescent speckles in the microtubules were visualized by adding \-rliodamine-labeled tithulin (~ l'7r "I total tnhulin in extract! anil using the CSU-IO confocal unit as described in the text. Arrows point to free microtubules extending from the spindle. iBl Kymograph showing the dynamics of fluorescent speckle flux. The kymograph is made up by displaying, on the horizontal axis, the fluorescence distribution along the black line in (A) us a function of time (vertical axis). The diagonal streaks are bright spots of fluorescent speckles which move over lime within the spindle, indicating mil rombiilc poleward flux. (C) Wide field fluorescence image of a different Xenopus extract spindle (~ 5% laheled-liihiilin). Notice the hoiiiogeneitv of the \pimlU: ID) Another Xenopus extract spindle <~ labeled-tubuliii) imaged by wide field fluorescence microscopy. Fluorescent speckles are visible, but finer details of the spindle are not visible due to fluorescence from oiit-of-focus planes. Bars: (A) and (B) = 10 : 1C) and (II) = 15 /ixm. back into mitosis by addition of a small amount of CSF-arrested extract. The resulting cytoplasmic extract contained metaphasc spindles (sperm nuclei were added for a source of chromatin) that were easily observed by addition of fluorescent labels (DAPI for DNA and x-rhodamine-labeled purified tubulin for microtubules). In previous studies, photo-uncaging of fluorescently labeled lubulin in spindles was used to show that there is a bulk flow of polymer originating near the spindle mid-zone and progressing toward each pole region. This How, termed microtubule poleward lluv was found to move at about 2 in Xenopus extract spindles; this is the same rate as the chromosome-to-pole move- ment during anaphase in this system. Therefore, it was pr
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
