. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. DISULFIDE PHO TOR EDUCTION 309 regenerates the disulfide and forms ATP. While this scheme is en- tirely speculative, it is backed by work with other related systems and chemical models (10-15). Our own work on inhibition of photo- phosphorylation by disulfide exchange processes suggested that coupling might take place through intervention of a sulfur-containing species (16). It was the initial immunochemical work that led to a search for an enzymatic system capable of photoreducing disulfides in the chroma- tophores of Rhodosp
. Bacterial photosynthesis. Bacteria -- Physiology; Photosynthesis. DISULFIDE PHO TOR EDUCTION 309 regenerates the disulfide and forms ATP. While this scheme is en- tirely speculative, it is backed by work with other related systems and chemical models (10-15). Our own work on inhibition of photo- phosphorylation by disulfide exchange processes suggested that coupling might take place through intervention of a sulfur-containing species (16). It was the initial immunochemical work that led to a search for an enzymatic system capable of photoreducing disulfides in the chroma- tophores of Rhodospirillum nihnim (17). Some recent experiments with this system have led to the view that the bacterial particles are capable of undergoing light-induced conformational changes, and that Disulfide Photoreduction. Time, Minutes Fig. 2. Photoreduction of the aromatic disulfide, DTNB, by Rhodospirillum mbnim chromatophores. The reaction mixtures contained in 3 ml. M tris buffer, pH , ml. chromato- phores ( 880 -â 40), 20/imoles ascorbate, ^mole dichloro- phenolindophenol, 2/ymoles methyl viologen, and 1 ^mole 5,5'- dithiobis-2-nitrobenzoic acid (DTNB). Ionic strength {jj) was al- tered by addition of (NH4)2SO^. Atmosphere, Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Symposium on Bacterial Photosynthesis (1963 : Yellow Springs, Ohio); Charles F. Kettering Research Laboratory (Yellow Springs, Ohio); Gest, Howard. Yellow Springs, Ohio, Antioch Press
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