. Carnegie Institution of Washington publication. BROWN ROT OP SOLANACEAE. I85 Result.—Very successful: The first signs appeared on the seventh to ninth day, beginning as brown streaks in many bitten places. On August 4, 49 separate infections were counted. August 14.—Twelve plants were inoculated: Potato, tomato, cucumber, and heliotrope. Potato culture No. 1, August 12, direct from the interior of plant No. 16, inoculated July 11, was used. Result.—Very successful on potato; less so on tomato. On cucumber a swollen stem appeared (the pricked internode); this contained living (motile) bacteri
. Carnegie Institution of Washington publication. BROWN ROT OP SOLANACEAE. I85 Result.—Very successful: The first signs appeared on the seventh to ninth day, beginning as brown streaks in many bitten places. On August 4, 49 separate infections were counted. August 14.—Twelve plants were inoculated: Potato, tomato, cucumber, and heliotrope. Potato culture No. 1, August 12, direct from the interior of plant No. 16, inoculated July 11, was used. Result.—Very successful on potato; less so on tomato. On cucumber a swollen stem appeared (the pricked internode); this contained living (motile) bacteria on September 8. Heliotrope (?). A ugust 17.—In this experiment 8 plants were inoculated: Portulaca oleraceae, tomato, Physalis ph iladelph ica, heliotrope, Citcurbita foetidissima, Eleusine indica, Ricinus communis,and Vigna catjang. Many pricks were made in each plant, introducing potato culture No. 1, August 12, which was direct from interior of plant No. 16, inoculated July 11. The tomato was punctured on a thrifty shoot near the top. Result.—Very successful on Physalis philadelpkica, the first sign appearing about the tenth day. For effect on Physalis see figs. 93, 94. The infection of tomato was slow—no wilt up to August 28. The other plants failed to contract the disease. August 20.—Ten inoculations were made on this date, using Solatium carolinense for the experiment. Many needle-pricks carried in large quantities of the bacterial culture (tube No. 1, August 12, as above), some of the pricks being made into leaves, others into the upper part of rather woody stems. Result.—All failed. Perhaps the inoculations were made too late in the season. The stems were woody. September 9.—Nine plants, part tomato, part potato, were inoculated from a fluid culture made directly from the swollen cucumber stem of August 14. Large numbers of bacteria were introduced by means of many needle-pricks. Result.—Negative. No records after September 21. Possibly the wrong o
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