. Plant anatomy from the standpoint of the development and functions of the tissues, and handbook of micro-technic. s filter this ripen about two months before using. To double stain the celloidin sections place them in safraninfor a day, rinse them in 50 per cent, alcohol, put them into MAKING PERMANENT MOUNTS 269 haematoxylin for about ten minutes, rinse them thoroughly inwater and then in 35 per cent, alcohol and again in 50 per cent,alcohol; pass them quickly through acid alcohol (one drophydrochloric acid in 50 of 70 per cent, alcohol), and thenput them through 70, 85, and
. Plant anatomy from the standpoint of the development and functions of the tissues, and handbook of micro-technic. s filter this ripen about two months before using. To double stain the celloidin sections place them in safraninfor a day, rinse them in 50 per cent, alcohol, put them into MAKING PERMANENT MOUNTS 269 haematoxylin for about ten minutes, rinse them thoroughly inwater and then in 35 per cent, alcohol and again in 50 per cent,alcohol; pass them quickly through acid alcohol (one drophydrochloric acid in 50 of 70 per cent, alcohol), and thenput them through 70, 85, and 95 per cent, alcohols, leaving themabout two minutes in each grade. Now clear the sections forabout two minutes in the mixture of equal parts of bergamotoil, cedar oil, and carbolic acid, and mount them in Canadabalsam. Here, as in all staining, the time ratios for the differentreagents will need to be determined for different materials. Making Permanent Mounts in Glycerine or GlycerineJelly.—Filamentous algae and fungi are pretty certain to shrinkand become plasmolyzed when put through the process of mount-. FIG. 146.—Turn table for cementing coverglasses to slides. For use where the mounting medium is glycerine or glycerine jelly. ing in balsam, but this danger can be easily avoided by mountingthem in glycerine or glycerine jelly, preferably the latter. Fixthe subjects in the chrom-acetic fixative described on page 260;wash them in running water for a few hours and place themin a per cent, aqueous solution of eosin for several hours;place for five minutes in a one per cent, solution of acetic acidin distilled water; wash out the acid completely in water andtransfer the material to a ten per cent, solution of glycerine; 270 PREPARATION OF SECTIONS tie a cloth over the dish to keep out the dust and allow the gly-cerine to concentrate by evaporation, and when it appearslike undiluted glycerine place the material in a drop of glycerineon a glass slip, put on a coverglass,
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