. Biological structure and function; proceedings. Biochemistry; Cytology. PHOTOSYNTHETIC PHOSPHORYLATION AND THE ENERGY CONVTIRSION PROCESS 383 TABLE XII Hydrogen Evolution from Reduced Methyl Violo(;en by Cell-Free Hydrogenase FROM Chrotnotiuni (Ogata, Nozaki, and Arnon, [91]) /(moles Ho evolved/ 10 chl. Complete system Methyl viologen omitted Na2S,04 omitted Hydrogenase omitted 12-8 none none none Complete systeni contained, in a final volume of 3-0 ml., cell-free suspension (PS) containing 0-4 mg. bacteriochlorophyll and 80 /nmoles tris buffer, pH 7-2. o-i ml. of 20",, KOH was
. Biological structure and function; proceedings. Biochemistry; Cytology. PHOTOSYNTHETIC PHOSPHORYLATION AND THE ENERGY CONVTIRSION PROCESS 383 TABLE XII Hydrogen Evolution from Reduced Methyl Violo(;en by Cell-Free Hydrogenase FROM Chrotnotiuni (Ogata, Nozaki, and Arnon, [91]) /(moles Ho evolved/ 10 chl. Complete system Methyl viologen omitted Na2S,04 omitted Hydrogenase omitted 12-8 none none none Complete systeni contained, in a final volume of 3-0 ml., cell-free suspension (PS) containing 0-4 mg. bacteriochlorophyll and 80 /nmoles tris buffer, pH 7-2. o-i ml. of 20",, KOH was present in the centre well and 16 ^imoles of methyl viologen was added to the sidearm. Methyl viologen was reduced by adding to the same sidearm while gassing with argon. The reaction was carried out at 30" in the dark. reactions as in the reduction of CO.^ via pyridine nucleotides or in the photofixation of N.,. The hydrogenase present in ChromoUuui particles that catalyzes hydrogen evolution in the light can also catalyze hydrogen gas evolution. text. LIGHT Non-cyclic electron transport in Chromatium Fig. 24. Scheme for non-cyclic electron flow in Chroniatiian. Details in the in the dark (Table XII) when electrons are supplied at a sufficiently reducing potential, as for example by hydrosulphite (cf. [132]). Methyl viologen was required as a catalyst in this reaction (compare [156, 1^7]).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
Size: 1533px × 1630px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookcollectionbiodiver, booksubjectbiochemistry
