. The Biological bulletin. Biology; Zoology; Marine biology. SPONGE CELL ADHESION 215. Figure 1. Aggregates showing partial covering of Microciona prolijcra by Halichondria bowerbanki aggregates in a suspension of mechanically dissociated cells. The darker aggre- gates are Microciona. aggregate where one or the other species was concentrated. A mixture of M and B cells with the B factor gave aggregation in only one out of nine trials, and this aggregate contained both M and B cells. Mixture of both cell types and both factors also resulted in the formation of common aggregates. A suspension of
. The Biological bulletin. Biology; Zoology; Marine biology. SPONGE CELL ADHESION 215. Figure 1. Aggregates showing partial covering of Microciona prolijcra by Halichondria bowerbanki aggregates in a suspension of mechanically dissociated cells. The darker aggre- gates are Microciona. aggregate where one or the other species was concentrated. A mixture of M and B cells with the B factor gave aggregation in only one out of nine trials, and this aggregate contained both M and B cells. Mixture of both cell types and both factors also resulted in the formation of common aggregates. A suspension of cells from both species failed to aggregate in the absence of either factor. These results are detailed in Table I. When cells of Halichondria panicea (P) are substituted for those of Hali- chondria bowerbanki, the results of the above experiments are far more erratic. Cross-aggregation of P by M factor occurred less often (three out of six trials), and the cells often appeared damaged. The combination of J/ and P cells with the M factor gave erratic results: sometimes the species formed intermingled aggregates and sometimes separate aggregates formed. It is probable that the factor isolation method used is not optimal for Halichondria with respect to both viability and potency of the factor obtained. Moscona (1968) established that the factor's enhancement of aggregation and its species-specificity in Microciona and Haliclona persisted even after the fixa- tion of the cells in formalin. The similarity of aggregation of fixed and living cells was also observed here. CMF-dissociated cells were fixed for four hours in 4% glutaraldehyde, washed in MBL-SW and combined as detailed above. The aggregates were much looser than those of living cells and less rounded, but the cells retained the same specific reactivity to factors as living cells. Mechanically dissociated cells Gvrationally aggregated mixtures of M and B cells, at either 4° C or room temperature, formed tight aggregates
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