. Carnegie Institution of Washington publication. I2O BACTERIA IN RELATION TO PLANT DISEASES. — If 3 at the left: Xylol, second xylol, xylol one-third absolute alcohol two-thirds, 95 per cent alcohol, 75 per cent alcohol, 55 per cent alcohol, 40 per cent alcohol, carbol- fuchsin, 40 per cent alcohol, second 40 per cent alcohol, 55 per cent alcohol, 65 per cent alcohol, 75 per cent alcohol, 95 per cent alcohol, absolute alcohol, second absolute alcohol, xylol, second xylol. From this last jar the material is mounted in balsam. Turpentine may be substituted for xylol in jars i and 2. After the p
. Carnegie Institution of Washington publication. I2O BACTERIA IN RELATION TO PLANT DISEASES. — If 3 at the left: Xylol, second xylol, xylol one-third absolute alcohol two-thirds, 95 per cent alcohol, 75 per cent alcohol, 55 per cent alcohol, 40 per cent alcohol, carbol- fuchsin, 40 per cent alcohol, second 40 per cent alcohol, 55 per cent alcohol, 65 per cent alcohol, 75 per cent alcohol, 95 per cent alcohol, absolute alcohol, second absolute alcohol, xylol, second xylol. From this last jar the material is mounted in balsam. Turpentine may be substituted for xylol in jars i and 2. After the paraffin is fully removed, the slides are passed rapidly from jar to jar (a minute or two in each being generally sufficient) until the stain is reached. After remain- ing in the stain the proper length of time (usually three to ten minutes, but sometimes much longer) the slides usually are allowed to remain in the 40 per cent alcohols for a number of minutes, with frequent inspection. When they appear to be properly bleached (rather pale) they are passed rapidly through the remaining jars until they reach the xylol, in which they may remain for some time without injury, if they can not be mounted immediately, but they must not be allowed to stand for any great length of time in any of the alcohols. The secret of success lies in obtain- ing just the proper amount of differentiation in the 40 per cent alcohol and in not losing any of this later Fig. 108.* . on. To retain the stain it is necessary sometimes to omit some of the graded alcohols. The time required for properly staining sections varies from one or t\vo minutes to a half day or more, according to the subject and the stain employed. No general rule applicable to all cases can be given. When the material is selected for embed- ding, its serial number, with a full description, is entered in the record book (fig. 1 12).. /" inS. ( )nr of a M-rie* of drawers divider! into small compartments for holding infiltrated, m
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