. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 8 16 32 40 TIME , min 64 72 80 - - O CO 2 B. o fO CM SS L/L/UUUUUUU. 10 20 30 40 TIME , min 50 60 60 50 UJ 405! 300 r- LU 20 < 10 0 Figure 2. Fractionation of a crude A' HCl extract from the nuclei of the sperm of Agnodesma saxicola. (A) Ionic exchange chromatography on a (10 X 100 mm) Protein-Pak SP 8HR column. Proteins were eluted with a linear (0-2 A/) NaCl gradient in 50 Na-phosphate buffer (pH ) at a flow rate of 1 ml/min. The inset shows the urea-acetic acid PAGE analy
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 8 16 32 40 TIME , min 64 72 80 - - O CO 2 B. o fO CM SS L/L/UUUUUUU. 10 20 30 40 TIME , min 50 60 60 50 UJ 405! 300 r- LU 20 < 10 0 Figure 2. Fractionation of a crude A' HCl extract from the nuclei of the sperm of Agnodesma saxicola. (A) Ionic exchange chromatography on a (10 X 100 mm) Protein-Pak SP 8HR column. Proteins were eluted with a linear (0-2 A/) NaCl gradient in 50 Na-phosphate buffer (pH ) at a flow rate of 1 ml/min. The inset shows the urea-acetic acid PAGE analysis of the fractions indicated. (B) Reverse-phase HPLC on a Vydac C4 column. Elution was carried with an acetonitrile gradient in trifluoroacetic acid at a flow rate of 1 ml/min. The inset shows the electrophoretic analysis of the fractionation. The lanes shown in the inset, and the chromatogram has been aligned to match the fraction analyzed with its corresponding position in the chromatogram. SS: starting Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
