. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 146 ERIK ZEUTHEN The fluid which fills the neck and bathes the eggs is a C(X binding buffer: glycyl- glycine m/10 or m/50 in sea water, with pH adjusted to by the addition of NaOH (pK of the ammo group in glycylglycine being according to Schmidt (1938, p. 613)). The buffer solution is made isotonic with sea water. During the division periods with which we are concerned (Fig. 2), the m/50 buffer was not observed to influence the development of the eggs at all, but in the m/10 buffer a slight retardation of the later
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 146 ERIK ZEUTHEN The fluid which fills the neck and bathes the eggs is a C(X binding buffer: glycyl- glycine m/10 or m/50 in sea water, with pH adjusted to by the addition of NaOH (pK of the ammo group in glycylglycine being according to Schmidt (1938, p. 613)). The buffer solution is made isotonic with sea water. During the division periods with which we are concerned (Fig. 2), the m/50 buffer was not observed to influence the development of the eggs at all, but in the m/10 buffer a slight retardation of the later cleavages (7-8) was noticed, and the blastulae did not swell quite normally. In the diver the oxygen bubble was kept down, close to the eggs, by the aid of a tiny glass extension on the stopper. The diver shown has the exact dimensions of the diver used in the experiment reproduced in Figure 2. The diver shown in Figure 1-B is almost identical with the one used for the experiments with Urechis, the main difference being that it floats with the stopper upwards. Figure 1-B gives the exact dimensions of the diver which was used in A 8 1mm buffer in sea w. a. NaOH /sot. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
