. Atlas of avian hematology. Veterinary hematology; Poultry -- Diseases. wheel. The first trial demonstrated that the par- ticles of emery and glass would completely plug the small bore of the glass tip. A thin-walled flexible rubber tubing was attached to the large end of the tip. An effort was made to dislodge the particles by blowing through the tubing dur- ing the grinding process. But blowing by mouth did not keep the bore free. It was found neces- sary to make a connection to an air or oxygen pressure tank in order to keep a flow of air or oxygen tliat would prevent the entrance of par-
. Atlas of avian hematology. Veterinary hematology; Poultry -- Diseases. wheel. The first trial demonstrated that the par- ticles of emery and glass would completely plug the small bore of the glass tip. A thin-walled flexible rubber tubing was attached to the large end of the tip. An effort was made to dislodge the particles by blowing through the tubing dur- ing the grinding process. But blowing by mouth did not keep the bore free. It was found neces- sary to make a connection to an air or oxygen pressure tank in order to keep a flow of air or oxygen tliat would prevent the entrance of par- ticles into the glass tip. The progress of grind- ing was checked under the microscope and was considered finished when there was a beveled, smooth, sharp tip. When the grinding was finished the tubes were cleaned with alcohol and ether, and dried with air. Method for taking blood from the dorsal aorta of the 48- to 72-hour embryo Eggs that are presumed to be fertile are taken from the cool room where they have been held at a temperature of about 55 degrees since they were laid. They are placed in the incubator with a record of the hour and date. In these studies the age has been taken as 3 hours less than the total time held in the incubator. When the embryo has reached 48 hours, incubation age (51 hours actual time in the incubator), it is removed, opened carefully, and slid into a bowl of warm saline or Ringer's solution. Sugiyama (1926) followed Sabin's suggestion and in- creased the salt content of Locke-Lewis solution to L04-percent NaCl for an embryo on the second day of incubation, to LO-percent for an embyro on the third day of incubation, and to for an embryo on the fourth day of incubation and older. These improvements in technic are useful if the embryos are to be held for study over a period of time. Wliere the whole procedure can be completed within a few mimites, the use of or solution of so- dium chloride alone produces no i
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