. A monograph of the British Uredineae and Ustilagineae, with an account of their biology including the methods of observing the germination of their spores and of their experimental culture. Smut fungi; Rust fungi; Smut diseases; Fungi. Spore-Ctilture. III place the spores in water, and they will germinate at once— for example, U. segetum, longissima, etc.; but some require a longer period of immersion, T. tritici, which will not germinate till after being several days in water. The germination of the Tilletia spores can also be conducted in a hanging-drop culture in the following manner
. A monograph of the British Uredineae and Ustilagineae, with an account of their biology including the methods of observing the germination of their spores and of their experimental culture. Smut fungi; Rust fungi; Smut diseases; Fungi. Spore-Ctilture. III place the spores in water, and they will germinate at once— for example, U. segetum, longissima, etc.; but some require a longer period of immersion, T. tritici, which will not germinate till after being several days in water. The germination of the Tilletia spores can also be conducted in a hanging-drop culture in the following manner:—A piece of glass tube, about half an inch (i2 mm.) in diameter and about three-quarters of an inch (i8 mm.) long, is cemented on an ordinary glass slide, so as to form a deep cell (Fig. lo).. Fig. lo.—Deep cell for hanging-drop cultureSj .made by cementing a piece of glass or lead Ltube upon an ordinary glass slide. Into this is placed a small quantity of water. The drop containing the spores to be germinated is placed on the centre of a circular , which will fit the top of the cell (Fig. II). If the upper edge of the tube which forms Fig, II.—Hanging-drop culture, seen in section. the cell be oiled, no air can get into the culture, and the germination can be watched for months, as the hanging- drop will not evaporate, because the water placed in the bottom of the cell keeps the atmosphere saturated, and any loss which it may sustain from evaporation is made up from the water at the bottom of the cell. There is no need to keep these sealed hanging-drop cultures in a moist atmosphere, because the necessary. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Plowright, Charles Bagge, 1849-1910. London, Paul, Trench & Co.
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Keywords: ., bookcentury1800, bookdecade1880, booksubjectfungi, booksubjectsmu
