. Pathological technique; a practical manual for workers in pathological histology and bacteriology. minutesto expel absorbed oxygen, cooled to 500 C, and then toeach tube is added 2 or 3 of sterile ascitic fluid which hasbeen previously heated to 60 ° C. for twenty-four hours. Thetubes are then cooled to 400 C. and inoculated as abovedescribed. In erysipelas the streptococcus is most readily found inthe extreme margin of the affected area where the process isnewest. The skin should be cleansed with soap and water,and with alcohol. Then with a sterile knife-point or a largeneedle a small
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. minutesto expel absorbed oxygen, cooled to 500 C, and then toeach tube is added 2 or 3 of sterile ascitic fluid which hasbeen previously heated to 60 ° C. for twenty-four hours. Thetubes are then cooled to 400 C. and inoculated as abovedescribed. In erysipelas the streptococcus is most readily found inthe extreme margin of the affected area where the process isnewest. The skin should be cleansed with soap and water,and with alcohol. Then with a sterile knife-point or a largeneedle a small wound should be made, and some of theblood and exudate pressed out from the tissue this cultures and cover-glasses may be prepared. Pneumococcus.—Synonyms: Diplococcus pneumoniae ;Micrococcus lanceolatus; Micrococcus of sputum-septi-cemia; Micrococcus pneumoniae crouposae. 264 PA THOL O GICAL TE CHNIQ UE. Morphology.—Pairs of rather small oval, conical, or lan-cet-shaped organisms, the broader ends being in organism varies somewhat in size, and one of the pair,:. Fig. 39.—Pneumococci with capsules in a cover-glass preparation from peri-cardial exudate stained by W. H. Smiths method ; X 1500 (W. H. Smith ; L. S. Brown). may be smaller than the other (Fig. 39). In some casesatypical or involution forms are seen, especially if the culturebe more than twenty-four hours old. No capsules are ordi-
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