Archive image from page 350 of Currents in biochemical research, 1956;. Currents in biochemical research, 1956; twenty-seven essays charting the present course of biochemical research and considering the intimate relationship of biochemistry to medicine, physiology, and biology currentsinbioche1956gree Year: 1956 ( ENZYME-SUBSTRATE COMPOUNDS Pushing Block Substrate syringe Substrate Stopcock for refilling _ substrate syringe Potentiometer for flow velocity Air vent Driving syrings Mixing chamber Observation chamber Stopping syringe Stop c Fig. 6. An apparatus for measuring the time sequen
Archive image from page 350 of Currents in biochemical research, 1956;. Currents in biochemical research, 1956; twenty-seven essays charting the present course of biochemical research and considering the intimate relationship of biochemistry to medicine, physiology, and biology currentsinbioche1956gree Year: 1956 ( ENZYME-SUBSTRATE COMPOUNDS Pushing Block Substrate syringe Substrate Stopcock for refilling _ substrate syringe Potentiometer for flow velocity Air vent Driving syrings Mixing chamber Observation chamber Stopping syringe Stop c Fig. 6. An apparatus for measuring the time sequence of oxidation of the components of the mitochondrial respiratory chain (24). Since a series of oxidations and reductions of the mitochondria can be carried out repetitively in this apparatus because exhaustion of the added oxygen occurs automatically with a respiratory system, the mitochondria can be used for a series of oxidations and reductions, and the apparatus is so ar- ranged to take advantage of this. Volume of the 'enzyme' syringe is 80 ml., 1 ml. for the 'substrate' syringe. Mixing time is less than 5 millisec. Satisfactory records can be obtained with cytochrome concentrations of less than 1 fxM (FA 21). 329
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