Practical physiological chemistry; a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . ing familiar with the use of the spectroscope. Howdoes the spectrum of oxyhemoglobin differ from that of the derived alkali hematin ?19. Preparation of Thrombin fHowell).—Prepare fibrin from pigs bloodaccording to directions given on page 268. Wash the fibrin thoroughly in waterto remove hemoglobin. Squeeze out the water, mince the fibrin and cover withan 8 per cent sodium chloride solution and allow to stand in the cold for 48 Precipi
Practical physiological chemistry; a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . ing familiar with the use of the spectroscope. Howdoes the spectrum of oxyhemoglobin differ from that of the derived alkali hematin ?19. Preparation of Thrombin fHowell).—Prepare fibrin from pigs bloodaccording to directions given on page 268. Wash the fibrin thoroughly in waterto remove hemoglobin. Squeeze out the water, mince the fibrin and cover withan 8 per cent sodium chloride solution and allow to stand in the cold for 48 Precipitate the thrombin fand other proteins j from the filtrate by addingan equal volume of acetone. Filter the mixture rapidly through a nximber ofsmall (25-50 ) filters. Spread out filter papers and precipitate and dry rapidlyin a current of cold air. Cut the dried papers into small pieces and treat with avolume of water eqmvalent to 66 per cent of the 8 per cent NaCl previously to stand one-half hour and filter. Shake the filtrate with chloroform(10-15 per 100 filtrate) until on settling no opalescenceis developed by. Fig. 80.—SiiDir-M Chloride. heating a portion of the supernatant fluid. Decant the Uquid and evaporateon watch glasses (2 to a watch glass) in a current of air. Thrombin so pre-pared may be kept indefinitely in a desiccator. 20. Variation in Size of Erythrocytes.—Prepare two small funnels with filterpapers such as are used in quantitative analysis. Moisten each paper with physio-logical (isotonic) salt solution. Into one funnel introduce a small amount ofdefibrinated ox blood and into the other funnel allow blood to drop directly from adecapitated frog. Note that the filtrate from the ox blood is colored, whereas thatfrom the frog blood is colorless. What deduction do you make regarding therelative size of the erythrocytes in ox and frog blood? Does either filtrate clot?Why? n. Blood Serum- I. Coagulation Temperature.—Place 5
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbiochem, bookyear1916
